Chiral Purity Test of Bevantolol by Capillaryelectrophoresis and High Performance Liquid Chromatography

다국어 초록 (Multilingual Abstract)

Two methods for the chiral purity determination of bevantolol were developed, namely capillary
electrophoresis (CE) using carboxymethyl-β-cyclodextrin (CM-β-CD) as a chiral selector
and high-perfomance liquid chromatography (HPLC) using a chiral stationary phase. In the
HPLC method, the separation of bevantolol enantiomers was performed on a Chiralpak AD-H
column by isocratic elution with n-hexane-ethanol-diethylamine (10:90:0.1, v/v/v) as mobile
phase. In the CE method, bevantolol enantiomers were separated on an uncoated fused silica
capillary with 50 mM amonium phosphate dibasic adjusted to a pH 6.5 with phosphoric acid
containing 15 mM CM-β-CD as running buffer. Validation data such as linearity, recovery,
detection limit, and precision of the two methods are presented. The detection limits of S-(-)-
bevantolol were 0.1% and 0.05% for CE and HPLC method, respectively and R-(+)-bevantolol
were 0.15% and 0.05% for CE and HPLC method, respectively. There was generally good
agreement between the HPLC and CE results.

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