<P>Porcine liver carboxylesterase (PLE) belongs to carboxylesterase family (EC 3.1.1.1) as a serine-type esterase. The PLE-catalyzed esterification of capric acid with glycerol in reverse micelles was investigated on the catalytic performance an...
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https://www.riss.kr/link?id=A107492490
Park, K.M. ; Lee, J.H. ; Hong, S.C. ; Kwon, C.W. ; Jo, M. ; Choi, S.J. ; Kim, K. ; Chang, P.S.
2016
-
SCOPUS,SCIE
학술저널
51-57(7쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P>Porcine liver carboxylesterase (PLE) belongs to carboxylesterase family (EC 3.1.1.1) as a serine-type esterase. The PLE-catalyzed esterification of capric acid with glycerol in reverse micelles was investigated on the catalytic performance an...
<P>Porcine liver carboxylesterase (PLE) belongs to carboxylesterase family (EC 3.1.1.1) as a serine-type esterase. The PLE-catalyzed esterification of capric acid with glycerol in reverse micelles was investigated on the catalytic performance and enzyme kinetics. The most suitable structure of reverse micelles was comprised of isooctane (reaction medium) and bis(2-ethylhexyl) sodium sulfosuccinate (AOT, anionic surfactant) with 0.1 of R-value ([water]/[surfactant]) and 3.0 of G/F-value ([glycerol]/[fatty acid]) for the PLE-catalyzed esterification. In the aspect of regio-selectivity, the PLE mainly produced 1-monocaprin without any other products (di- and/or tricaprins of subsequent reactions). Furthermore, the degree of esterification at equilibrium state (after 4 h from the initiation) was 62.7% under the optimum conditions at pH 7.0 and 60 degrees C. Based on Hanes-Woolf plot, the apparent K-m and V-max values were calculated to be 16.44 mM and 38.91 mu M/min/mg protein, respectively. (C) 2015 Elsevier Inc. All rights reserved.</P>
Improved PCR performance and fidelity of double mutant Neq A523R/N540R DNA polymerase