Efficacy of Nile tilapia (Oreochromis niloticus) juveniles and spinosyns bioinsecticides against aquatic stages of Culex pipiens: An experimental study

다국어 초록 (Multilingual Abstract)

The mosquito Culex pipiens is the most widely distributed dipteran species in all regions of Egypt and the principal vector of Wuchereria bancrofti and certain arboviruses in human beings. For controlling C. pipiens vector, biological tools (e.g., larvivorous fish and bioinsecticides) are more potent and safer options to the environment, human beings, and beneficial organisms than chemical pesticides. The efficiency of O. niloticus juveniles as predatory fish species and two bioinsecticides, spinosad 24% and spinetoram 12%, was investigated against the C. pipiens developmental stages in the laboratory. The first trial evaluated the predatory efficacy of small-sized O. niloticus (2.1–2.6 cm; 250–315 mg) and large-sized O. niloticus (2.5–3.2 cm; 250–315 mg) against the 3rd larvae and pupae of C. pipiens. This is the first report in Egypt confirming the predation potential of O. niloticus as efficient predatory fish against the immature C. pipiens. Large-sized O. niloticus predated a greater number of 3rd of C. pipiens larvae and pupae than the small-sized ones. Furthermore, the daily consumption of C. pipiens larvae by small- and large-sized O. niloticus was significantly higher than the pupae. The second trial assessed the toxicity efficacy of spinosad 24% and spinetoram 12% against C. pipiens larvae and pupae. The results confirmed that the tested bioinsecticides showed higher potency toward C. pipiens larvae than pupae after exposure for 24 h and 48 h. Spinosad was more toxic toward 3rd C. pipiens larvae (LC 50 = 0.013 and 0.003 mg/L) and pupae (LC 50 = 320.69 and 44.28 mg/L) than spinetoram after 24 and 48 h. Herein, O. niloticus juveniles (as promising native predatory fish) and spinosyns bioinsecticides were more effective against C. pipiens in the larval stage than in the pupal stage. In conclusion, Nile tilapia juveniles and biorational compounds, spinosad 24% and spinetoram 12%, might be considered as promising and favorable environmental biological agents for controlling C. pipiens in Egypt. However, further trials are needed to investigate the potential of these agents in the control of this mosquito vector under field conditions.

참고문헌 (Reference)

1 Manna, B., "Vulnerability of the mosquito larvae to the guppies(Poecilia reticulata)in the presence of alternative preys" 45 : 200-206, 2008

2 Surendran, S. N., "Use of tilapia, Oreochromis mossambicus, for the control of mosquito breeding in water storage tanks in the Jaffna district of Sri Lanka" 36 : 107-110, 2008

3 Gad, A. M., "Uptake and development of Wuchereria bancrofti in Culex pipiens L. and Aedes caspius pallas" 26 : 305-314, 1996

4 Mohamed, I. A., "Unique efficacy of certain novel herbicides against Culex pipiens(Diptera : Culicidae)larvae mosquito under laboratory conditions" 10 : 104-111, 2016

5 Pereira, B. B., "Toxicological assessment of spinosad : Implications for integrated control of Aedes aegypti using larvicides and larvivorous fish" 79 : 477-481, 2016

6 dos Santos Dias, L., "Toxicity of spinosad to temephos-resistant Aedes aegypti populations in Brazil" 12 : e0173689-, 2017

7 Sun, Y. -P., "Toxicity Index-an improved Method of comparing the relative 378Toxicity of Insecticides" 43-, 1950

8 Elbarky, N. M., "Toxicicological evaluation and biochemical impacts for radient as a new generation of spinosyn on Spodoptera littoralis (Boisd.) larvae" 1 : 85-97, 2008

9 Matias, J. R., "The use of annual killifish in the biocontrol of the aquatic stages of mosquitoes in temporary bodies of fresh water; A potential new tool in vector control" 3 : 46-, 2010

10 Bond, J. G., "The naturally derived insecticide spinosad is highly toxic to Aedes and Anopheles mosquito larvae" 18 : 50-56, 2004

11 Bahgat, I., "The natural bioinsecticide spinosad and its toxicity to combat some mosquito species in Ismailia Governorate, Egypt" 3 : 396-400, 2007

12 Mbuya, N., "The influence of juvenile fish(Oreochromis niloticus)on population density of pond breeding mosquitoes in the degraded Wetlands of Yala Swamp, Western Kenya" 1 : 59-71, 2014

13 Kramer, V., "The ecology and biological control of mosquitoes [Culex tarsalis, Anopheles freeborni and A. franciscanus [A. pseudopunctipennis franciscanus]] in California wild and white rice fields [using Bacillus thuringiensis and larvivorous fish]" 49 : 1989

14 Wu, N., "The advantages of mosquito biocontrol by stocking edible fish in rice paddies" 22 : 436-442, 1991

15 Elghareeb, T. A., "Synergistic action of glyphosate on novel pesticides against Culex pipiens L. (Diptera:Culicidae) mosquitoes under laboratory conditions" 12 : 45-52, 2018

16 Kamatchi, P. A. C., "Study of mosquito control using larvivorous fish Danio rerio Hamilton and Oreochromis mossambicus Peters" 4 : 8-9, 2016

17 Salgado, V. L., "Studies on the mode of action of spinosad : insect symptoms and physiological correlates" 60 : 91-102, 1998

18 P´erez, C., "Spinosad, a naturally derived insecticide, for control of Aedes aegypti(Diptera : Culicidae) : efficacy, persistence, and elicited oviposition response" 44 : 631-638, 2007

19 Marina, C. F., "Spinosad : a biorational mosquito larvicide for use in car tires in southern Mexico" 5 : 95-, 2012

20 Sparks, T. C., "Resistance and crossresistance to the spinosyns – a review and analysis" 102 : 1-10, 2012

21 Das, M., "Native larvivorous fish diversity as a biological control agent against mosquito larvae in an endemic malarious region of Ranchi district in Jharkhand, India" 55 : 34-41, 2018

22 Chandra, G., "Mosquito control by larvivorous fish" 127 : 13-, 2008

23 Howard, A. F., "Malaria mosquito control using edible fish in western Kenya : preliminary findings of a controlled study" 7 : 199-, 2007

24 Ghosh, S. K., "Larvivorous fish against malaria vectors : a new outlook" 101 : 1063-1064, 2007

25 Kumar, R., "Larvicidal efficiency of aquatic predators : a perspective for mosquito biocontrol" 45 : 447-466, 2006

26 Prabhu, K., "Larvicidal and pupicidal activity of spinosad against the malarial vector Anopheles stephensi" 4 : 610-613, 2011

27 Michaelakis, A., "Larvicidal activity of spinosad and its impact on oviposition preferences of the West Nile vector Culex pipiens biotype molestus–a comparison with a chitin synthesis inhibitor" 74 : 101917-, 2020

28 Chatterjee, S., "Laboratory trials on the feeding pattern of Anopheles subpictus, Culex quinquefasciatus and Armigeres subalbatus by Xenentodon cancila fry" 14 : 173-174, 1996

29 Collins, A. P., "Laboratory evaluation of the freshwater prawn, Macrobrachium borellii, as a predator of mosquito larvae" 60 : 22-27, 1998

30 Darriet, F., "Laboratory evaluation of pyriproxyfen and spinosad, alone and in combination, against Aedes aegypti Larvae" 43 : 1190-1194, 2006

31 Bhattacharjee, I., "Laboratory and field assessment of the potential of larvivorous, air-breathing fishes as predators of culicine mosquitoes" 49 : 126-133, 2009

32 Norman-Lopez, ´ A., "Is tilapia the same product worldwide or are markets segmented?" 13 : 138-154, 2009

33 Williams, T., "Is the naturally derived insecticide Spinosad®compatible with insect natural enemies?" 13 : 459-475, 2003

34 Kumar, P. M., "Integration of botanical and bacterial insecticide against Aedes aegypti and Anopheles stephensi" 112 : 761-771, 2013

35 Wang, C. H., "Integrated control of the dengue vector Aedes aegypti in Liu-Chiu village, Ping-Tung County, Taiwan" 16 : 93-99, 2000

36 Martinez-Ibarra, J., "Indigenous fish species for the control of Aedes aegypti in water storage tanks in Southern Mexico" 47 : 481-486, 2002

37 Ghosh, A., "Functional response and density dependent feeding interaction of Oreochromis niloticus against immatures of Culex quinquefasciatus" 54 : 366-368, 2017

38 Darriet, F., "Field evaluation of pyriproxyfen and spinosad mixture for the control of insecticide resistant Aedes aegypti in Martinique(French West Indies)" 3 : 88-, 2010

39 Marcombe, S., "Field efficacy of new larvicide products for control of multiresistant Aedes aegypti populations in Martinique(French West Indies)" 84 : 118-126, 2011

40 Bibi, S., "Evaluation of wild tilapia and gift tilapia as biological control against mosquito larvae(Culex quinquefasciatus and Aedes aegypti)" 4 : 23-27, 2017

41 Cetin, H., "Evaluation of the naturally-derived insecticide spinosad against Culex pipiens L. (Diptera: Culicidae) larvae in septic tank water in Antalya, Turkey" 30 : 151-154, 2005

42 Abebe, A., "Efficacy of tilapia, Oreochromis niloticus and Tilapia Zilli for the control of mosquito larvae around Fincha Valley, Oromia region" 5-, 2018

43 Marina, C. F., "Efficacy of larvicides for the control of dengue, Zika, and chikungunya vectors in an urban cemetery in southern Mexico" 117 : 1941-1952, 2018

44 Halwart, M., "Efficacy of common carp and Nile tilapia as biocontrol agents of the golden apple snail in the Philippines" 143 : 125-136, 2014

45 Paarlberg, T., "Effectiveness and residual speed of flea kill of a novel spot on formulation of spinetoram(Cheristin®)for cats" 10 : 59-, 2017

46 El Kady, G. A., "Comparative toxicity of two bio-insecticides(Spinotoram and Vertemic)compared with methomyl against Culex pipiens and Anopheles multicolor" 4 : 198-205, 2008

47 Ghosh, A., "Biological control of vector mosquitoes by some common exotic fish predators" 29 : 167-171, 2005

48 Chala, B., "Assessment of Clarias gariepinus as a biological control agent against mosquito larvae" 16 : 27-, 2016

49 Halwart, M., "Activity pattern of fish in rice fields" 145 : 159-170, 1996

50 Watson, G. B., "Actions of insecticidal spinosyns on γ-aminobutyric acid responses from small-diameter cockroach neurons" 71 : 20-28, 2001

51 Hertlein, M. B., "A review of spinosad as a natural product for larval mosquito control" 26 : 67-87, 2010

52 Yıldırım, O., "A preliminary study on determination of Aphanius chantrei’s feeding behaviour on mosquito larvae" 102 : 172-175, 2007