대두 단백질 분해효소 억제제의 cDNA 유전자 분리 = A cDNA Cloning of Soybean Proteinase Inhibitor

다국어 초록 (Multilingual Abstract)

A cDNA clone for the soybean trypsin inhibitor(Kunitz-type: SKTI) was isolated by immunological screening of a soybean lamda gtll expression vector cDNA library with antibody. The cDNA insert size of the clone lamda Ti-4 was 308 bp. Nucleotide sequencing of the clone shows open reading frame of 201 bp which encodes 67 amino acids of the SKTI precursor and 107 bp of 3'-nontranslating region including a part of the poly(A) tail. Comparision of deduced amino acid sequence reveals that the clone Ti-4 was partial cDNA clone of SKTI. SKTI precursor contains a stretch of 11 amino acid at C-terminal suggesting that the precursor is processed at C-terminal to produce mature SKTI in soybean seeds. Northeran blot analysis demonstrated the mRNA size of SKTI to be about 900 bp. It was expressed only in soybean seeds suggesting tissue specific gene expression was regulated at the level of transcription.