Protective Effects of SAPP, a Novel Herbal Complex, in Acute Hepatotoxic Mouse Model

다국어 초록 (Multilingual Abstract)

The protective effect of SAPP, an extract from a novel herbal complex, on acute liver injury was investigated using mouse animal model in this study. The content of total phenol in SAPP was increased at dose dependent manner. Consistent with the content of total phenol, SAPP showed the significant anti-oxidative effects on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) method. Acute liver injury was induced by D-galactosamine (D-GalN) in mouse. Treatment with SAPP significantly reduced the level of alanine transaminase (ALT) and aspartate transaminase (AST) in serum. Histological observation revealed that whereas D-GalN treated mouse showed vacuolization of hepatocytes, sinusoidal dilation and congestion, loss of cell boundaries and ballooning degeneration, loss of architecture and cell necrosis, treatment with SAPP improved D-GalN-induced liver injury. These results suggest that SAPP shows protective effects against D-GalN-induced hepatotoxicity in vivo acute mouse model.

참고문헌 (Reference)

1 서경순, "인진(茵蔯)으로 쓰이는 사철쑥과 더위지기 추출물의 항산화 활성" 한국자원식물학회 21 (21): 292-298, 2008

2 조우철, "오리나무 추출물(AI-1367)의 간질환 동물모델에서의 간 보호효과" 대한약학회 56 (56): 99-107, 2012

3 최혁재, "순무의 d-galactosamine 유발 간장해 보호효과" 한국생약학회 37 (37): 258-265, 2006

4 Park, E. J., "The ethanolsoluble part of a hot-water extract from Artemisia iwayomogi inhibits liver fibrosis induced by carbon tetrachloride in rats" 52 : 875-881, 2000

5 Lee, T. Y., "The effect of capillarisin on glycochenodeoxycholic acid-induced apoptosis and heme oxygenase-1 in rat primary hepatocytes" 325 : 53-59, 2009

6 Tsai, T. H., "Simultaneous blood and biliary sampling of esculetin by microdialysis in the rat" 65 : 1647-1655, 1999

7 Al-Anati, L., "Silibinin protects OTA-mediated TNF-alpha release from perfused rat livers and isolated rat Kupffer cells" 53 : 460-466, 2009

8 Whang, H. J., "Quantitative analysis of totalphenolic content in apple" 14 : 377-383, 2001

9 Choi, J. H., "Protective effects of hyperoside against carbon tetrachloride-induced liver damage in mice" 74 : 1055-1060, 2011

10 He, C. S., "Protective effects of capillary artemisia polysaccharide on oxidative injury to the liver in rats with obstructive jaundice" 4 : 645-648, 2012

11 Li, X. H., "Phenolic constituent of Phellodendron chinense Bark" 87 : 1218-1221, 2009

12 Marzouk, M. S., "Pharmacologically active ellagitannins from Terminalia myriocarpa" 68 : 523-527, 2002

13 Maeda, S., "Pharmacological studies on schizandra fruits. II. Effects of constituents of schizandra fruits on drugs induced hepatic damage in rats" 102 : 579-588, 1982

14 Dong-Wook Kim, "Isorhamnetin-3-O-galactoside Protects against CCl4-Induced Hepatic Injury in Mice" 한국응용약물학회 20 (20): 406-412, 2012

15 Yuan, L., "Isoorientin induces apoptosis through mitochondrial dysfunction and inhibition of PI3K/Akt signaling pathway in HepG2 cancer cells" 265 : 83-92, 2012

16 Choi, W. S., "Inhibitory effect on proliferation of vascular smooth muscle cells and protective effect on CCl(4)-induced hepatic damage of HEAI extract" 100 : 176-179, 2005

17 Jayaraj, R., "Hepatoprotective efficacy of certain flavonoids against microcystin induced toxicity in mice" 22 : 472-479, 2007

18 Mak, D. H., "Effects of schizandrin B and alphatocopherol on lipid peroxidation, in vitro and in vivo" 165 : 161-165, 1996

19 Ip, S. P., "Effect of schizandrin B on hepatic glutathione antioxidant system in mice: protection against carbon tetrachloride toxicity" 62 : 398-401, 1995

20 Seeff, L. B., "Complementary and alternative medicine in chronic liver disease" 34 : 595-603, 2001

21 이위, "Chemical Constituents from the Bark of Phellodendron amurense and Their Cytotoxic Effects on HL-60 Human Leukemia Cells" 한국생약학회 18 (18): 250-253, 2012

22 이영미, "Aqueous Extract of Schizandra chinensis Suppresses Dextran Sulfate Sodiuminduced Generation of IL-8 and ROS in the Colonic Epithelial Cell Line HT-29" 한국생약학회 15 (15): 185-191, 2009

23 Gordon, M. H., "Antioxidant activity of hydroxytyrosol acetate compared with that of other olive oil polyphenols" 49 : 2480-2485, 2001

24 Hikino, H., "Antihepatotoxic actions of lignoids from Schizandra chinensis fruits" 50 : 213-218, 1984

25 Wang, J. H., "Antifibrotic effects of Artemisia capillaris and Artemisia iwayomogi in a carbon tetrachloride-induced chronic hepatic fibrosis animal model" 140 : 179-185, 2012

26 Kim, A. R., "Active components from Artemisia iwayomogi displaying ONOO(-) scavenging activity" 18 : 1-7, 2004