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      인간배아줄기세포 유래 배상체내의 신경세포군 확인과 분화과정 동안 이뤄지는 신경발생과정의 이해 = Detection of Neural Cell Population in Embryoid Body Derived from Human Embryonic Stem Cells and Understanding of Neural Developmental Stage during Differentiation

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      다국어 초록 (Multilingual Abstract)

      Recently, human embryonic stem cells(hESCs) have been highlighted to understand early embryogenesis as well as to apply to clinical cell therapy. During human embryogenesis, dynamic processes of early neuronal development are very important to elucidate their developing mechanism. However, the mechanism has not been entirely clear. The formation of embryoid bodies(EBs) derived from hESCs is a tissue-like spheroids in suspension culture and has a potential to differentiate into the three germ layers. Even though many kinds of specific cell makers have been studied, the specific proteins expressed from differentiated cells have been elusive. The objective of this study is to investigate the expression of neuronal protein while EBs is differentiating from hESCs(day 5, 10, 15 and 20). The expressions of neuronal specific makers such as Neurofilament 68kDa(NF 68), tubulin III(TuJ-1) and microtubule-associated protein 2(MAP2) in each EBs stage were analyzed by immunofluorescence(IF). The expressions and localization of neuronal specific markers were shown to be different depending on EBs stages. NF 68 positive cells were expressed on the surface of EBs at day 10 and the expression of NF68 almost disappeared at day 20. MAP2 was not detected in EBs at day 10, whereas it began to be slightly detected on the surface of EBs at day 15. On the other hand, TuJ-1 positive cells were found at all stages and their expression levels were maintained until day 20. These results demonstrate neuronal specific markers can be expressed differently depending on EBs stages, suggesting this allows us to probably understand the early development of neurogenesis in hESCs. Furthermore, this idea expands that we are able to understand the development of all specific cells differentiated from early human embryogenesis.
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      Recently, human embryonic stem cells(hESCs) have been highlighted to understand early embryogenesis as well as to apply to clinical cell therapy. During human embryogenesis, dynamic processes of early neuronal development are very important to elucida...

      Recently, human embryonic stem cells(hESCs) have been highlighted to understand early embryogenesis as well as to apply to clinical cell therapy. During human embryogenesis, dynamic processes of early neuronal development are very important to elucidate their developing mechanism. However, the mechanism has not been entirely clear. The formation of embryoid bodies(EBs) derived from hESCs is a tissue-like spheroids in suspension culture and has a potential to differentiate into the three germ layers. Even though many kinds of specific cell makers have been studied, the specific proteins expressed from differentiated cells have been elusive. The objective of this study is to investigate the expression of neuronal protein while EBs is differentiating from hESCs(day 5, 10, 15 and 20). The expressions of neuronal specific makers such as Neurofilament 68kDa(NF 68), tubulin III(TuJ-1) and microtubule-associated protein 2(MAP2) in each EBs stage were analyzed by immunofluorescence(IF). The expressions and localization of neuronal specific markers were shown to be different depending on EBs stages. NF 68 positive cells were expressed on the surface of EBs at day 10 and the expression of NF68 almost disappeared at day 20. MAP2 was not detected in EBs at day 10, whereas it began to be slightly detected on the surface of EBs at day 15. On the other hand, TuJ-1 positive cells were found at all stages and their expression levels were maintained until day 20. These results demonstrate neuronal specific markers can be expressed differently depending on EBs stages, suggesting this allows us to probably understand the early development of neurogenesis in hESCs. Furthermore, this idea expands that we are able to understand the development of all specific cells differentiated from early human embryogenesis.

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      참고문헌 (Reference)

      1 SW Cho,

      2 김상성, "저산소 배양조건에서 인간배아줄기세포의 발생경향" 한국조직공학과 재생의학회 5 (5): 474-481, 2008

      3 김주미, "인간배아 줄기세포로부터 분화된 혈관내피세포의 분리 및 in vivo 실험을 통한 조직재생 가능성 평가" 한국조직공학과 재생의학회 3 (3): 451-456, 2006

      4 김주미, "hESC로부터 유래된 혈관내피전구세포를 위한 최적의 배양조건 확립" 한국조직공학과 재생의학회 5 (5): 467-473, 2008

      5 T Barberi, "Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice" 21 : 1200-, 2003

      6 KD Deb, "Human embryonic stem cells: preclinical perspectives" 6 : 7-, 2008

      7 I Klimanskaya, "Human embryonic stem cells derived without feeder cells" 365 : 1636-, 2005

      8 T Dvash, "Human embryonic stem cells as a powerful tool for studying human embryogenesis" 60 : 111-, 2006

      9 BNI Friedrich, "Human embryonic stem cells as a cellular model for human disorders, Mol Cell Figure 4. Expression pattern of Neuronal cell markers in hEBs during neuronal differentiation" 252 : 154-, 2006

      10 I Klimanskaya, "Human embryonic stem cell lines derived from single blastomeres" 444 : 481-, 2006

      1 SW Cho,

      2 김상성, "저산소 배양조건에서 인간배아줄기세포의 발생경향" 한국조직공학과 재생의학회 5 (5): 474-481, 2008

      3 김주미, "인간배아 줄기세포로부터 분화된 혈관내피세포의 분리 및 in vivo 실험을 통한 조직재생 가능성 평가" 한국조직공학과 재생의학회 3 (3): 451-456, 2006

      4 김주미, "hESC로부터 유래된 혈관내피전구세포를 위한 최적의 배양조건 확립" 한국조직공학과 재생의학회 5 (5): 467-473, 2008

      5 T Barberi, "Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice" 21 : 1200-, 2003

      6 KD Deb, "Human embryonic stem cells: preclinical perspectives" 6 : 7-, 2008

      7 I Klimanskaya, "Human embryonic stem cells derived without feeder cells" 365 : 1636-, 2005

      8 T Dvash, "Human embryonic stem cells as a powerful tool for studying human embryogenesis" 60 : 111-, 2006

      9 BNI Friedrich, "Human embryonic stem cells as a cellular model for human disorders, Mol Cell Figure 4. Expression pattern of Neuronal cell markers in hEBs during neuronal differentiation" 252 : 154-, 2006

      10 I Klimanskaya, "Human embryonic stem cell lines derived from single blastomeres" 444 : 481-, 2006

      11 J Yamashita, "Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors" 2 : 43-, 2000

      12 S Levenberg, "Endothelial cells derived from human embryonic stem cells" 4391-, 2002

      13 G Bain, "Embryonic stem cells express neuronal properties in vitro" 168 : 342-, 1995

      14 JA Thomson, "Embryonic stem cell lines derived from human blastocysts" 282 : 1145-, 1998

      15 J Kim, "Effective isolation and culture of endothelial cells in embryoid body differentiated from human embryonic stem cells" 16 : 269-, 2007

      16 박제원, "Chondrogenic differentiation of human embryonic stem cells by hSOX9 overexpression" 한국조직공학과 재생의학회 5 (5): 109-116, 2008

      17 NL Boyd, "BMP4 promotes formation of primitive vascular networks in human embryonic stem cell-derived embryoid bodies" 232 : 833-, 2007

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      학술지 이력

      학술지 이력
      연월일 이력구분 이력상세 등재구분
      학술지등록 한글명 : 조직공학과 재생의학
      외국어명 : Tissue Engineering and Regenerative Medicine
      2023 평가예정 해외DB학술지평가 신청대상 (해외등재 학술지 평가)
      2020-01-01 평가 등재학술지 유지 (해외등재 학술지 평가) KCI등재
      2013-10-01 평가 등재학술지 선정 (기타) KCI등재
      2012-01-01 평가 등재후보 1차 FAIL (기타) KCI등재후보
      2011-01-01 평가 등재후보 1차 PASS (등재후보1차) KCI등재후보
      2010-01-01 평가 등재후보 1차 FAIL (등재후보1차) KCI등재후보
      2008-01-01 평가 SCIE 등재 (신규평가) KCI등재후보
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      학술지 인용정보

      학술지 인용정보
      기준연도 WOS-KCI 통합IF(2년) KCIF(2년) KCIF(3년)
      2016 1.08 0.42 0.81
      KCIF(4년) KCIF(5년) 중심성지수(3년) 즉시성지수
      0.69 0.51 0.367 0.03
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