rRNA Spacer 부위를 이용한 Candida의 균종동정 = Identification of Candida Species by Analysis of rRNA Spacer Regions

다국어 초록 (Multilingual Abstract)

Background: The genus Candida comprises 163 species, the most common pathogen in the genus is C. albicans, however, other Candida species are considered as emergent pathogens. Because there are species-specific differences in the susceptibility of Candida spp. to the currently used therapeutic drugs, species identification is critical for therapeutic planning and accurate epidemiological records. The objective of this study is to evaluate the performance of tRNA intergenic length polymorphism (tDNA-ILP) analysis for the accurate identification of Candida species.
Methods: For the identifying the 7 type strains and 54 clinical isolates of Candida, the suitability of tDNA-ILP was evaluated. The polymerase chain reaction (PCR) using a pair of primers or a single primer was performed. The PCR products were separated by electrophoresis in 2% agarose gels for 70 min.
Results: In seven Candida type strains, tDNA-ILP using a single primer (reverse) can be easily analyzed by visual comparision. Fifty-three of 54 strains were identified as the same species with conventional identification.
Conclusions: The tDNA-ILP analysis can be useful for the simple and rapid identification of Candida species in routine laboratories.

참고문헌 (Reference)

1 "유전자종 분석을 이용한 Acinetobacter의 균종동정" 20 : 48-55, 2000

2 "Use of molecular methods in identification of Candida species and evaluation of fluconazole resistance" 98 : 1027-1032, 2003

3 "The epide- miology of candidemia in two United States cities:results of a population-based active surveillance" 29 : 1164-1170, 1999

4 "The Ins and Outs of DNA Fingerprinting the Infectious Fungi" 13 : 332-370, 2000

5 "Susceptibility testing of fluconazole by the NCCLS broth macrodilution method,E-test,and disk diffusion for application in the routine labora- tory" 40 : 918-921, 2002

6 "Species differentiation by internally transcribed spacer PCR and HhaI digestion of fluconazole-resistant Candida krusei,Candida inconspicua,and Candida norvegensis strains" 35 : 1036-1039, 1997

7 "Significance of amplified fragment length polymorphism in identification and epidemio- logical examination of Candida species colonization in children undergoing allogeneic stem cell transplan- tation" 42 : 1673-1679, 2004

8 "Rapid species identification of coagulase negative Staphylococci by rRNA spacer length polymorphism analysis" 189-194, 2001

9 "Rapid identifi- cation and fingerprinting of Candida krusei by PCR based amplification of the species-specific repetitive polymorphic sequence CKRS-1" 35 : 1337-1343, 1997

10 "Rapid iden- tification of Candida species with species-specific DNA probes" 36 : 3260-3265, 1998

11 "Rapid detection and identification of Candida,Aspergillus,and Fusarium species in ocular samples using nested PCR" 38 : 2902-2908, 2000

12 "Principles and applications of methods for DNA-based typing of microbial orga- nisms" 37 : 1661-1669, 1999

13 "PCR-based identification of pathogenic Candida sp- ecies using primer mixes specific to Candida DNA topoisomerase II genes" 19 : 973-989, 2002

14 "PCR-amplified length poly- morphisms in tRNA intergenic spacers for categori- zing staphylococci" 6 : 1673-1680, 1992

15 "Opportunistic candi- diasis:an epidemic of the 1980s" 21 : 897-904, 1995

16 "Molecular probes for diagnosis of fungal infections" 33 : 2913-2919, 1995

17 "Mole- cular epidemiological study of Aspergillus fumigatus in a bone marrow transplantation unit by PCR ampli- fication of ribosomal intergenic spacer sequences" 36 : 1294-1299, 1998

18 "Identification of species of the genus Candida by analysis of the 5.8S rRNA gene and the two ribo- somal internal transcribed spacers" 85 : 175-185, 2004

19 "Human mycoses:drugs and targets for emerging pathogens" 264 : 371-373, 1994

20 "Fingerprinting genomes using PCR with arbitary primers" 18 : 7213-7218, 1990

21 "Econo- mic impact of Candida colonization and Candida infection in the critically ill patient" 23 : 323-330, 2004

22 "Differentiation and species identi- fication of yeasts using PCR" 48 : 279-286, 1998

23 "DNA polymorphisms amplified by arbi- tary primers are useful as genetic markers" 18 : 1531-1635, 1990

24 "DNA fingerprinting of medically im- portant microorganisms by use of PCR" 7 : 174-184, 1994

25 "Construction and use of PCR primers from a 65 kDa mannoprotein gene for identification of C.albicans" 18 : 171-175, 2004

26 "Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast species" 42 : 2209-2211, 2004

27 "Antifungal resis- tance in non-albicans Candida species" 2 : 9-14, 1999