생물학적 살선충제의 뿌리혹선충 (Meloidogyne incognita) 방제 효과

국문 초록 (Abstract)

친환경 선충 방제제 개발을 위해 경북 성주군 선남면 및 충남 공주군 우성면의 시설원예 재배지 토양으로 부터 선충 포식성이 뛰어난 곰팡이 Arthrobotrys thaumasia Nema-1과 선충 표피성분인 collagen과 알집 주성분인 gelatin 분해능이 뛰어난 Bacillus subtilis C-9를 분리 하였다. 이들 분리균을 대상으로 포트실험을 통해 선충치사 효과의 지표인 난낭수 감소를 검토한 결과, 균밀도가 7.0 × 10<SUP>3</SUP> cfu g<SUP>-1</SUP> 인 A. thaumasia Nema-1 곰팡이 제제 5,000 mg kg<SUP>-1</SUP>을 처리시 뿌리혹 선충의 난낭수가 무처리 대비 35% 감소하였다. 포식성 곰팡이 Nema-1 제제와 균밀도가 8.5 × 10<SUP>5</SUP> cfu g<SUP>-1</SUP> 인 B. subtilis C-9 세균 제제 각각 5,000 mg kg<SUP>-1</SUP> 혼합 처리구에서는 난낭수가 무처리 대비 67% 감소하였다. 또한 선충치사효과를 증진시키기 위하여 살선충 활성이 있다고 보고된 계피추출물 제제 10 mg kg<SUP>-1</SUP>을 5,000 mg kg<SUP>-</SUP>의 Nema-1과 C-9 제제와 혼합하여 처리 하였을 때 난낭수가 무처리 대비 84%이상 감소하였으며, 대표적 살선충제인 선충탄(Fosthiazate) 24 mg kg<SUP>-1</SUP>은 난낭수가 26% 감소한 결과와 비교해 볼 때 훨씬 높은 수준이었다. 이상의 결과는 생물학적 선충방제제는 미생물 또는 식물체 추출물 단제 보다는 혼합물 형태로 사용하는 것이 더 효과적이라는 결과를 제시하였다.

다국어 초록 (Multilingual Abstract)

An nematophagous fungi Arthrobotrys thaumasia Nema-1 and Bacillus subtilis C-9, which degrade the collagen and gelatin, were isolated from horticulture plantation soil in Kyungpook Sungju-gun Seonnam-myun and Chungnam Gongju-gun Woosung-myun to develop biological nematode pesticide. When 5,000 mg kg<SUP>-1</SUP> of A. thaumasia Nema-1 nematicide powder (7.0 × 10³ cfu g<SUP>-1</SUP>) was treated to pot including Meloidogyne incognita, the number of nematode’ egg mass, which is a index of nematicidal activity, decreased to 35% compared to control. While the number of nematode’ egg mass decreased to 67% by treating the nematicide powder mixture of 5,000 mg kg<SUP>-1</SUP> Nema<SUP>-1</SUP> and B. subtilis C-9 (8.5 × 10<SUP>5</SUP> cfu g<SUP>-1</SUP>). Furthermore the number of nematode’ egg mass of the mixture containing cinnamon extract 10 mg kg<SUP>-1</SUP>, each 5,000 mg kg<SUP>-1</SUP> of Nema<SUP>-1</SUP> and C-9 nematicide powder was decreased to 84%, comparing to the result showed the number of nematode’ egg mass decreased to 24%, by the treatment of chemical nemato pesticide Fosthiazate 24 mg kg<SUP>-1</SUP>. These results suggested the mixture of microorganisms and plant extract was more effective biological nematicide than the case of only microorganism or plant extract for nematode control.

목차 (Table of Contents)

• 서언
• 재료 및 방법
• 결과
• 고찰
• 요약
• 인용문헌

참고문헌 (Reference)

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