Rapid analysis of drugs of abuse and their metabolites in human urine using dilute and shoot liquid chromatography–tandem mass spectrometry

다국어 초록 (Multilingual Abstract)

Liquid chromatography-tandem mass spectrometricmethod for analysis of 113 abuse drugs and theirmetabolites in human urine was developed and validated. Asimple sample clean-up procedure using the ‘‘dilute andshoot’’ approach, followed by reversed phase separation,provided a fast and reliable method for routine analysis.
Drugs were separated in a Capcell Pak MG-III C18 columnusing a gradient elution of 1 mM ammonium formate with0.1% formic acid in water and acetonitrile. The total timefor analysis was 32 min. The multiple reaction monitoringmode using two transitions (e.g., quantifier and qualifier)was optimized for both identification and determination.
The calibration curves for each analyte were linear over theconcentration ranges of 1–100, 5–100, or 10–100 ng/mLusing 400 lL of human urine sample with the coefficient ofdetermination above 0.9921. The coefficient of variationand accuracy for the intra- and inter-assays of the testeddrugs at three QC levels were 1.1–14.6 and 86.7–106.8%,respectively. The present method was successfully appliedto the analysis of forensic urine samples obtained from 17drug abusers. This method is useful for the rapid andaccurate determination of multiple drug abuse with a smallamount of urine in forensic and clinical toxicology.

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