The vector for gene manipulation of Rhizobium, pGUR9, was constructed by combining the mobilization and broad host range replication function of RSF1010 with E. coli cloning vehicle, pACYC184. The Rhizobium vectors, pGUR15 and pGUR19 were also constru...
The vector for gene manipulation of Rhizobium, pGUR9, was constructed by combining the mobilization and broad host range replication function of RSF1010 with E. coli cloning vehicle, pACYC184. The Rhizobium vectors, pGUR15 and pGUR19 were also constructed by combining indigenous plasmid pASR286 and pASR186 from Acacia Rhizobium with pSUP104 and pABR322, respectively. These vectors were stably maintained in a variety of Rhizobium and Agrobacterium. They can be efficiently mobilized by RP4 tra function and have selecton markers and versatile cloning sites of the E. coli vectors. They can be efficiently transfered to Rhizobium by transformation.