미량오염물질에 의한 항생제 내성 유전자 전이 특성에 대한 실험모델 개발 = The Experimental Model Development of Antibiotic Resistance Gene Transfer Characteristics with Various Micropollutants

다국어 초록 (Multilingual Abstract)

Recently, antibiotic resistant genes (ARGs) in the environment are emerging as pollutants, since these genetic contaminants can eventually be transferred to human pathogens. The aim of this study was to develop the experimental model of antibiotic resistant gene (ARG) plasmid transfer as a function of various environmental conditions. For this purpose, the multi drug resistant plasmid pB10, which is known to be originally isolated from a wastewater treatment plant, was selected as a model transfer plasmid and Escherichia coli DH5α containing pB10 was used as a model donor. Pseudomonas aeruginosa, an opportunistic pathogen, was selected as the recipient for the conjugation experiment. When the donor and recipient were exposed to various stressors including antibiotics and heavy metal as a function of the concentrations (10, 100 and, 1000 ppb), statistically increased plasmid transfer rate was observed at a concentration of 10 ppb of tetracycline and sulfamethoxazole compared to control (no antibiotic exposure). Accordingly, the developed experimental ARG model by various stressor is a promising tool for evaluating the dissemination of ARGs by micro-contaminants in aquatic environment.

참고문헌 (Reference)

1 Park. J. H., "환경 내항생제 내성세균 및 유해성에 관한 국내조사현황" 40 : 150-157, 2008

2 황대호, "화옹호의 부영양화 방지를 위한 영양염류 삭감률 산정" 한국물환경학회 20 (20): 589-596, 2004

3 김응석, "한국형 부영양화 지수를 이용한 저수지 수질평가" 한국물환경학회 28 (28): 78-83, 2012

4 정현미, "상수원에서 바이러스와 분변오염지표세균의 계절변동" 한국물환경학회 19 (19): 329-338, 2003

5 오향균, "다양한 물 환경시료에서 항생제 내성 미생물 유해성 검출을 위한 배양 분리방법 개발" 대한토목학회 27 (27): 711-717, 2007

6 Barr, V., "β-Lactam Antibiotics Increase the Frequency of Plasmid Transfer in Staphylococcus Aureus" 17 : 409-413, 1986

7 Forbes, B. A., "Transfer of Resistance Plasmids from Staphylococcus Epidermidis to Staphylococcus Aureus: Evidence for Conjugative Exchange of Resistance" 153 : 627-634, 1983

8 Satoshi, S., "Transfer of Antibiotic Multi Resistant Plasmid RP4 from Escherichia coli to Activated Sludge Bacteria" 106 : 292-296, 2008

9 Bonheyo, G. T., "Transfer Region of a Bacteroides Conjugative Transposon Contains Regulatory as Well as Structural Genes" 46 : 202-209, 2001

10 Guardabassi, L., "The Effects of Tertiary Wastewater Treatment on the Prevalence of Antimicrobial Resistant Bacteria" 36 : 1955-1964, 2002

11 Kim, S., "The Effect of Operational Parameters on the Fate of Tetracycline Resistant Bacteria in Biological Wastewater Treatment Plants" State University of New York 2005

12 Schluter, A., "The 64508 bp IncP-1β Antibiotic Multi Resistance Plasmid pB10 Isolated from a Waste Water Treatment Plant Provides Evidence for Recombination between Members of Different Branches of the IncP-1β Group" 149 : 3139-3153, 2003

13 Guardabassi, L., "Occurrence and Fate of Antibiotic Resistant Bacteria in Sewage" Danish Environmental Protection Agency 2002

14 Bonot, S., "Monitoring the Dissemination of the Broad-Host-Range Plasmid pB10 in Sediment Microcosms by Quantitative PCR" 76 : 378-382, 2010

15 Sambrook, J., "Molecular Cloning: A Laboratory Manual, 3rd Edition" Cold Spring Harbor Laboratory Press 2001

16 Coleman, M. E., "Microbial Risk Assessment Scenarios, Causality, and Uncertainty" 2 : 13-17, 2007

17 Ohlsen, K., "Impact of Antibiotics on Conjugational Resistance Gene Transfer in Staphylococcus Aureus in Sewage" 5 : 711-716, 2003

18 Levy, S. B., "Factors Impacting on the Problem of Antibiotic Resistance" 49 : 25-30, 2002

19 Knapp, C., "Evidence of Increasing Antibiotic Resistance Gene Abundances in Archived Soils Since 1940" 44 : 580-587, 2010

20 김성표, "Enhanced biodegradation of iopromide and trimethoprim in nitrifying activated sludge" AMER CHEMICAL SOC 40 (40): 7367-7373, 200612

21 Livermore, D., "Antimicrobial Resistance; Inevitable, but not Unmanageable" Health Protection Agency (HPA) 4-11, 2005

22 Fajardo, A., "Antibiotics as Signals that Trigger Specific Bacterial Responses" 11 : 161-167, 2008

23 Reinthaler, F. F., "Antibiotic Resistance of E.coli in Sewage and Sludge" 37 : 1685-1690, 2003