Shigella의 鷄胎感染에 關한 硏究 = Experimental Shigella Infection in the Embryonated Eggs

다국어 초록 (Multilingual Abstract)

The present study was initiated to extend observation of shigella virulence in the chick embryo and to evaluate: (ⅰ) the effect of route of inoculation and age of embryos on the virulence of shigella strains, (ⅱ) the effect of variation among shigella strains on their virulence for the chick embryo, which would permit comparisons of virulence based on LD_(50) determinations, (ⅲ) the lethal effect of endotoxin of shigella on the chick embryos, (ⅳ) the growth rates at the site of inoculation and other various sites on infected chick embryos, and (ⅴ) to obtain some observations which might relate to the shigella strains for chick embryos.
The studies were carried out for virulence of representative shigella strain (Sh. dysenteriae 1, Sh. flexneri 4a, Sh. boydii 3, and Sh. sonnei 1) on 9-, 13-, and 15-day chick embryos by various route of inoculation with serial dilutions of viable and nonviable inocula (heat-killed cell, and cultural filtrates).
The author observed that the susceptibility of younger embryos (9-day) decreased with increasing embryonic maturity(13-, 15-day), and 9-day old embryo is maximally susceptible regardless of the route of inoculation, but 15-day old embryos has shown susceptibility in both routes of intravenous and chorioallantoic membranes. These findings indicate that the route of inoculation appeared to be highly correlated with virulence for chick embryos.
The growth curves of organisms inoculated allantoically showed maximal multiplication in allantoic fluid regardless of susceptibility.
The lethal effect of representative shigella strain was shown to be most virulent by Sh. dysenteriae 1, and Sh. flexneri 4a, Sh. sonnei 1, and Sh. boydii 3 in order by inoculation of chorioallantoic membrane route.
The endotoxin of Sh. dysenteriae 1 was destroyed completely by heating at 120℃ for 20 minutes.
Taken together, these findings indicate that chick embryo may serve as a suitable host in which to measure virulence of shigella.

목차 (Table of Contents)

• Ⅰ. 緖論
• Ⅱ. 實驗方法 및 材料
• Ⅱ-1. 供試菌株
• Ⅱ-2. 孵化卵
• Ⅱ-3. 接種物
• Ⅱ-4. 接種方法
• Ⅱ-5. 生菌數計算
• Ⅱ-6. 接種孵化卵의 血中尿酸値測定
• Ⅲ. 實驗成績
• Ⅲ-1. 接種經路別 孵化卵의 Sh. dysenteriae 1에 對한 感受性
• Ⅲ-2. Shigella의 各血淸型別 鷄胎菌力
• Ⅲ-3. 接種經路別 感染菌의 部位別 增殖像
• Ⅲ-4. 感染卵의 血中尿酸値의 變動
• Ⅲ-5. 培養濾液, 加熱死菌 및 加熱死菌濾液의 鷄胎致死에 미치는 溫度의 影響
• Ⅳ. 總括 및 考察
• Ⅴ. 結論
• 文獻