Immobilization of Heparin to Galactosylated Chitosan as a Synthetic Extracellular Matrix for Liver Tissue Engineering

다국어 초록 (Multilingual Abstract)

Galactose moiety was covalently coupled with chitosan to improve hepatocyte adhesion and heparin was immobilized
to the galactosylated chitosan (GC) to enhancing hepatocyte function. Hepatocyte adhesion to heparin immobilized
GC (GCH)-casted polystyrene (PS) dish was higher than that to chitosan-casted PS dish although it was
slightly lower than GC-casted PS dish owing to the hydrated GCH-casted PS dish. Spreading of hepatocytes
attached onto GCH-casted PS dish was increased with an increase of heparin for the GCH in the presence of hepatocyte
growth factor (HGF). Also, DNA synthesis of the hepatocyte adhered to GCH-casted PS dish was increased
with an increase of heparin in the GCH. Higher levels of albumin secretion were maintained on the GCH-casted
PS dish than chitosan- or GC-casted PS dishes.

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