Differential Expression Screening of Defense Related Genes in Vitis flexuosa Grapevine against Elsinoe ampelina and Rhizobium vitis

다국어 초록 (Multilingual Abstract)

To collect useful genetic resources and information for disease resistant grape breeding from Korean wild grape, various genes related with defense responses were screened for their differential expressions in Vitis flexuosa grapevines against Elsinoe ampelina and Rhizobium vitis. The expressions of plant defense-related genes such as thaumatin-like protein (TLP), glutathione peroxidase (GPX), glutathione-S-transferase (GST), chalcone synthesis (CHS), protein kinase regulator (14-3-3), and â-glucanase-like protein (Glu), and signal transduction-related genes such as leucine reach-repeat (LRR) and lipoxygenase (LOX), and cell wall modification-related gene such as polygalacturonase-inhibiting protein (PGIP) were induced by E. ampelina inoculation. WRKY transcription factor 10 (WRKY), fatty acid elongase (FAE), tonoplast intrinsic protein (TIP), and meiosis 5 (Mei5) were up-regulated and proline-rich protein (PRPs) were down-regulated by both inoculation of spores and treatment of culture filtrates (CF) of E. ampelina. Among 15 up-regulated genes, LRR, LOX, TLP, and GST were specifically up-regulated by spore inoculation than CF treatment. On the contrary, CHS and TIP were highly upregulated by CF treatment than spore inoculation of E. ampelina. While LRR, CLP, LOX, TLP, GPX, 14-3-3, GST, PGIP, FAE, TIP, Glu, Mei5, and WRKY were up-regulated, CHS and PRPs were downregulated by R. vitis inoculation. The defense genes related with active oxygen species such as GPX and GST were highly activated in grapevine leaves with inoculated R. vitis.

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