히스타민 및 항히스타민제가 P_(388) 백혈병세포계증식에 미치는 영향에 관한 실험적연구 = Effect on Histamine and Antihistamine on P_(388) Leukemic Cell

다국어 초록 (Multilingual Abstract)

Histamine, one of the major mediators in IgE-mediated anaphylaxis, has also effect both in humoral and cellular immuue reaction, such as proliferation of effector or suppressor T lymphocytes, antibody formation and production of the macrophage migration inhibition factor.
Such effects of histamine are known to be mediated by H₂ receptor located on the surface of lymphocytes and inhibited by H₂ receptor antagonist. Function of histamine and H₂-receptor antagonist as an immunomodulator enabled them to be tried to obtain an indirect anticancer effect.
However, direct of histamine or H₂-receptor antagonist on growth of tumor cell is unknown.
To understand direct effect of histamine or H₂-receptor antagonist on growth of tumor cell, histamine and H2-receptor antagonist (cimetidine) were added in Fischer's culture media in concentration of 10^(-3)M, 10^(-4)M and 10^(-5)M and cultured P_(383) leukemic cell for 24, 48 and 72 hours.
The results are as followings;
1. Histamine revealed inhibitory activity to the growth of P_(383) cell line. And the activity revealed dosage dependency on histamine.
2. H₂-receptor antagonist showed no inhibitory effect on growth of P_(383) cell line.
3. Inhibitory effect of histamine of P_(383) cell line was not blocked by Cimetidine.
Above results suggest that histamine has inhibitory effect on growth of P_(383) leukemic cell lice and this activity seems to have no relationship with H₃-receptor.