한우의 ACADS 유전자내의 SNP 탐색 및 경제형질과의 연관성 분석

다국어 초록 (Multilingual Abstract)

The acyl-CoA dehydrogenase, C-2 to C-3 short chain (ACADS) gene is known to be related with fat metabolism, especially coverts the fat to the energy sources in cattle. In human, the mutations in this gene cause SCAD deficiency, which is one of the fatty acid metabolism disorders. The ACADS gene is located on bovine chromosome 17. The objective of this study was to identify SNPs in Hanwoo ACADS gene and identify the relationships with economic traits. In this study, two SNPs, T1570G SNP in exon 2 and G13917A SNP in exon 4, were observed. Moreover, in the coding region, 2 missense mutations, T (Cys) → G (Trp) mutation at 1570 bp and G (Arg) → A (Gln) mutation at 13917 bp, were observed. These mutations were subjected to the PCR-RFLP for typing 198 Hanwoo animals. The observed genotype frequency for T1570G was 0.135 (TT), 0.860 (TG) and 0.005 (GG), respectively. Also, 0.900 (GG) and 0.100 (GA) were observed for the G13917A mutation. The association of these SNPs with four economic traits, CW (Carcass Weight), BF (Backfat Thickness), LMA (Longissimus Muscle Area), MS (Marbling Score), were also observed. The results indicated that no significant results were observed in all four traits (P＞0.05). This might indicate that further studies are ultimately needed to use the SNPs in ACADS gene in lager populations for effectively used for the marker assisted selection.

목차 (Table of Contents)

• Abstract
• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• Ⅲ. 결과 및 고찰
• Ⅳ. 결론
• 감사의 글
• 참고문헌

참고문헌 (Reference)

1 오재돈, "한우(Bos taurus coreanae)의 CAST 유전자 내 변이지역 탐색 및 경제형질과의 연관성 분석" 한국생명과학회 20 (20): 1498-1504, 2010

2 Hoequette JF, "The future trends for research on quality and safety of animal product" 4 : 49-72, 2005

3 Tein I, "Short-chain acyl-CoA dehydrogenase gene mutation (c.319C-T) presents with clinical heterogeneity and is candidate founder mutation in individuals of Ashkenazi Jewish origin" 93 : 179-189, 2008

4 Corydon MJ, "Role of common gene variations in the molecular pathogenesis of short-chain acyl-CoA dehydrogenase deficiency" 49 : 18-23, 2001

5 Seidel J, "Recurrent vomiting and ethylmalonic aciduria associated with rare mutations of the short-ethylmalonic aciduria associated with rare mutations of the short-chain acyl-CoA dehydrogenase gene" 26 : 37-42, 2003

6 Nonneman D, "Rapid communication: a polymorphic microsatellite in the promoter region of the bovine calpastatin gene" 77 : 3114-3115, 1999

7 Morris CA, "Quantitative trait loci for live animal and carcass composition traits in Jersey and Limousin backcross cattle finished on pasture or feedlot" 40 (40): 648-654, 2009

8 Nkrumah JD, "Primary genome scan to identify putative quantitative trait loci for feedlot growth rate, feed intake, and feed efficiency of beef cattle" 85 (85): 3170-1381, 2007

9 Marques E, "Polymorphisms in positional candidate genes on BTA14 and BTA26 affect carcass quality in beef cattle." 87 (87): 2475-2484, 2009

10 Clark DL, "Muscle gene expression associated with increased marbling in beef cattle" 22 (22): 51-63, 2011

11 Shirao K, "Molecular pathogenesis of a novel mutation, G108D, in short-chain acyl-CoA dehydrogenase identified in subjects with short-chain acyl-CoA dehydrogenase deficiency" 127 : 619-628, 2010

12 Tatum JD, "Interrelationships between marbling, subcutancous fat thickness, and cooked beef palatability" 43 : 777-784, 1982

13 Naito Y, "Identification of two variant short chain acyl-coenzyme A dehydrogenase alleles, each containing a different point mutation in a patient with short chain acyl-coenzyme A dehydrogenase deficiency" 85 : 1575-1582, 1990

14 Seung-Hwan Lee, "Identification of marbling-related candidate genes in M. longissimus dorsi of high- and low marbled Hanwoo (Korean Native Cattle) steers" 한국생화학분자생물학회 41 (41): 846-851, 2008

15 Gregersen N, "Identification of four new mutations in the short-chain acyl-CoA dehydrogenase (SCAD) gene in two patients: one of the variant alleles, 511C→T, is present at an unexpectedly high frequency in the general plpulation, as was the case for 625G→A, together conferring susceptibility to ethylmalonic aciduria" 7 : 619-627, 1998

16 Morris CA, "Genotypic effects of calpain 1 and calpastatin on the tenderness of cooked M. longissimus dorsi steaks from Jersey x Limousin, Angus and Hereford-cross cattle" 37 (37): 411-414, 2006

17 Sherman EL, "Fine mapping quantitative trait loci for feed intake and feed efficiency in beef cattle" 87 (87): 37-45, 2009

18 Moore SS, "Fine mapping of quantitative trait loci and assessment of positional candidate genes for backfat on bovine chromosome 14 in a commercial line of Bos taurus" 81 : 1919-1925, 2003

19 Wu XL, "Evaluation of candidate gene effects for beef backfat via Bayesian model selection" 125 (125): 103-113, 2005

20 Casas E, "Effects of calpastatin and micro-calpain markers in beef cattle on tenderness traits" 84 (84): 520-525, 2006

21 Geay Y, "Effect of nutritional factors on biochemical, structural and metabolic factors on of muscles in ruminant, consequences on dietic value and sensorial qualities of meat" 41 : 1-26, 2001

22 Casas E, "Detection of quantitative trait loci for growth and carcass composition in cattle" 81 (81): 2976-2983, 2003

23 Kim JJ, "Detection of quantitative trait loci for growth and beef carcass fatness traits in a cross between Bos taurus (Angus) and Bos indicus (Brahman) cattle" 81 (81): 1933-1942, 2003

24 Vockley J, "Defects of mitochondrial betaoxidation: a growing group of disorders" 12 : 235-246, 2002

25 Thaller G, "DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle" 34 : 354-357, 2003

26 vanMaldegem BT, "Clinical, biochemical, and genetic heterogeneity in short-chain acyl-coenzymeA dehydrogenase deficiency" 296 : 943-952, 2006

27 Ferraz JB, "Association of single nucleotide polymorphisms with carcass traits in Nellore cattle" 8 (8): 1360-1366, 2009

28 Schenkel FS, "Association of single nucleotide polymorphisms in the leptin gene with carcass and meat quality traits of beef cattle" 83 (83): 2009-2020, 2005

29 Schenkel FS, "Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle" 84 (84): 291-299, 2006

30 Buchanan FC, "Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels" 34 : 105-116, 2002

31 Morsci NS, "Association analysis of adiponectin and somatostatin polymorphisms on BTA1 with growth and carcass traits in Angus cattle" 37 (37): 554-562, 2006

32 Barendse W., "Assessing lipid metabolism. International patent application PCT/AU98/00882"

33 Jiao Y, "A novel polymorphism of the MYPN gene and its association with meat quality traits in Bos taurus." 9 (9): 1751-1758, 2010

34 McClure MC, "A genome scan for quantitative trait loci influencing carcass, post-natal growth and reproductive traits in commercial Angus cattle" 41 (41): 597-607, 2010

35 Casas E, "A comprehensive search for quantitative trait loci affecting growth and carcass composition of cattle segregating alternative forms of the myostatin gene" 79 (79): 854-860, 2001