Monocyte Attachment and Migration through Collagen IV in Diabetes Mellitus

다국어 초록 (Multilingual Abstract)

The interactions between monocytes and extracellular
matrix proteins have been implicated in atherosclerosis
pathophysiology. In the present study we evaluated
monocyte attachment and migration through oxidized
and non-oxidized collagen IV. Monocyte attachment
was tested on microwells coated with either native or
oxidized collagen IV. Monocyte migration through collagen
IV was examined on transwells. Monocytes derived
from patients with diabetes mellitus showed an
increased ability to attach and migrate through collagen
IV as compared to those derived from healthy volunteers.
Moreover, control monocytes attached to oxidized
collagen at a higher degree, while they migrated
through oxidized collagen at a lower degree, as compared
to the native protein. Our results also showed the
involvement of the alpha2 integrin subunit in the above
phenomena suggesting a modified interaction between
monocytes and collagen IV in diabetes mellitus.

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