RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      KCI등재 SCOPUS

      과산화수소의 산화적 스트레스로 유도된 Apoptosis에 대한 생약복합조성물(HemoHIM)의 방호효과 평가

      한글로보기

      https://www.riss.kr/link?id=A76301978

      • 0

        상세조회
      • 0

        다운로드
      서지정보 열기
      • 내보내기
      • 내책장담기
      • 공유하기
      • 오류접수

      부가정보

      국문 초록 (Abstract)

      본 연구팀에서는 방사선으로부터 위장관과 면역조혈계를 방호하기 위하여 당귀, 천궁, 백작약으로 새로운 생약복합물 HIM-I을 개발하였고, HIM-I을 에탄올 침전하여 에탄올 분획(HIM-I-E)과 조다당 분획(HIM-I-P)을 얻은 후, HIM-I에 조다당 분획을 첨가하여 HemoHIM을 제조하여 실험하였으며, 과산화수소를 이용하여 산화적 스트레스를 유발한 후 HemoHIM의 apoptosis 억제 효과를 측정하였다. 과산화수소를 세포주에 처리한 결과 세포독성 증가, 세포막 파괴, subG1기 세포 증가, DNA 절단 현상 등이 관찰되었다. 이때 HIM-I, HIM-I-P, HemoHIM을 첨가하여 전처리 배양한 다음에 과산화수소로 apoptosis를 유도하였다. 그 결과 과산화수소에 의한 세포독성, 세포막 파괴, subG1기 세포, DNA 절단현상 등이 현저하게 감소하는 것으로 나타났다. 전반적으로 HemoHIM의 방호 효과는 HIM-I-P와 비슷하였으며 HIM-I보다 높은 것으로 나타났다. 이상의 결과로 HemoHIM은 면역기능 방호?회복 물질로서 뿐만 아니라 산화적 스트레스에 대한 항산화제로서 유용하게 활용될 수 있을 것으로 생각된다.
      번역하기

      본 연구팀에서는 방사선으로부터 위장관과 면역조혈계를 방호하기 위하여 당귀, 천궁, 백작약으로 새로운 생약복합물 HIM-I을 개발하였고, HIM-I을 에탄올 침전하여 에탄올 분획(HIM-I-E)과 조다...

      본 연구팀에서는 방사선으로부터 위장관과 면역조혈계를 방호하기 위하여 당귀, 천궁, 백작약으로 새로운 생약복합물 HIM-I을 개발하였고, HIM-I을 에탄올 침전하여 에탄올 분획(HIM-I-E)과 조다당 분획(HIM-I-P)을 얻은 후, HIM-I에 조다당 분획을 첨가하여 HemoHIM을 제조하여 실험하였으며, 과산화수소를 이용하여 산화적 스트레스를 유발한 후 HemoHIM의 apoptosis 억제 효과를 측정하였다. 과산화수소를 세포주에 처리한 결과 세포독성 증가, 세포막 파괴, subG1기 세포 증가, DNA 절단 현상 등이 관찰되었다. 이때 HIM-I, HIM-I-P, HemoHIM을 첨가하여 전처리 배양한 다음에 과산화수소로 apoptosis를 유도하였다. 그 결과 과산화수소에 의한 세포독성, 세포막 파괴, subG1기 세포, DNA 절단현상 등이 현저하게 감소하는 것으로 나타났다. 전반적으로 HemoHIM의 방호 효과는 HIM-I-P와 비슷하였으며 HIM-I보다 높은 것으로 나타났다. 이상의 결과로 HemoHIM은 면역기능 방호?회복 물질로서 뿐만 아니라 산화적 스트레스에 대한 항산화제로서 유용하게 활용될 수 있을 것으로 생각된다.

      더보기

      다국어 초록 (Multilingual Abstract)

      In our previous study, a novel herb mixture (HIM-I) of Angelica gigas radix, Cnidium officinale rhizoma, and Paeonia japonica radix was developed to protect the intestinal and immune systems and promote its recovery against radiation damage. A new herbal composition (HemoHIM) with the high immune modulating activity was developed from HIM-I. HIM-I was fractionated into ethanol fraction (HIM-I-E) and polysaccharide fraction (HIM-I-P). And HemoHIM was prepared by adding HIM-I-P to HIM-I. HemoHIM showed more effective than HIM-I in immune modulation as well as radioprotection. The present study is designed to investigate the protective effects of HIM-I, HIM-I-P, and HemoHIM on hydrogen peroxide (H₂O₂)-induced apoptosis of human promyelocytic leukemia (HL-60) cells. It was shown that H₂O₂ treatment reduced the viability of cells, and increased appearance of DNA ladders, hypodiploid (subG1) cells, and phosphatidylserine translocation level. Pretreatment of HemoHIM significantly reduced the cytotoxic effect induced by H₂O₂, associated with reducing the translocation of phosphatidylserine, hypodiploid cells and DNA ladders. HemoHIM appeared to be more protective than HIM-I against H₂O₂-induced apoptosis whereas, it exhibited similar activity to HIM-I-P. These results indicated that HemoHIM might be an useful agent for protection against oxidative stress (H₂O₂)-induced apoptosis as well as immune modulation, especially since it is a relatively nontoxic natural product.
      번역하기

      In our previous study, a novel herb mixture (HIM-I) of Angelica gigas radix, Cnidium officinale rhizoma, and Paeonia japonica radix was developed to protect the intestinal and immune systems and promote its recovery against radiation damage. A new her...

      In our previous study, a novel herb mixture (HIM-I) of Angelica gigas radix, Cnidium officinale rhizoma, and Paeonia japonica radix was developed to protect the intestinal and immune systems and promote its recovery against radiation damage. A new herbal composition (HemoHIM) with the high immune modulating activity was developed from HIM-I. HIM-I was fractionated into ethanol fraction (HIM-I-E) and polysaccharide fraction (HIM-I-P). And HemoHIM was prepared by adding HIM-I-P to HIM-I. HemoHIM showed more effective than HIM-I in immune modulation as well as radioprotection. The present study is designed to investigate the protective effects of HIM-I, HIM-I-P, and HemoHIM on hydrogen peroxide (H₂O₂)-induced apoptosis of human promyelocytic leukemia (HL-60) cells. It was shown that H₂O₂ treatment reduced the viability of cells, and increased appearance of DNA ladders, hypodiploid (subG1) cells, and phosphatidylserine translocation level. Pretreatment of HemoHIM significantly reduced the cytotoxic effect induced by H₂O₂, associated with reducing the translocation of phosphatidylserine, hypodiploid cells and DNA ladders. HemoHIM appeared to be more protective than HIM-I against H₂O₂-induced apoptosis whereas, it exhibited similar activity to HIM-I-P. These results indicated that HemoHIM might be an useful agent for protection against oxidative stress (H₂O₂)-induced apoptosis as well as immune modulation, especially since it is a relatively nontoxic natural product.

      더보기

      목차 (Table of Contents)

      • Abstract
      • 서론
      • 재료 및 방법
      • 결과 및 고찰
      • 요약
      • Abstract
      • 서론
      • 재료 및 방법
      • 결과 및 고찰
      • 요약
      • 감사의 글
      • 문헌
      더보기

      참고문헌 (Reference)

      1 "The role of proteases during apoptosis" 10 : 587-597, 1996

      2 "The role of AP-1, NF-kappaB and ROS/NOS in skin carcinogenesis: the JB6 model is predictive." 234 : 185-193, 2002

      3 "The radioprotective effects of radices herbs" 41 : 105-111, 2001

      4 "The radioprotective effects of Bu- Zhong-Yi-Qi-Tang A prescription of traditional Chinese medicine" 30 : 127-137, 2002

      5 "The radioprotective effect of Kuei-Pi- Tang as a prescription of traditional Chinese medicine in mice" 28 : 698-704, 1999

      6 "The effects of herbs on the radiation-induced apoptosis in intestinal crypt cells" 26 : 27-33, 2001

      7 "The effects of Shi-Quan-Dai-Bu-Tang and its ingredients on the survival of jejunal crypt cells and hematopoietic cells in irradiated mice" 29 : 93-98, 2000

      8 "The control of apoptosis in mammalian cells" 307-309, 1993

      9 "Structurally related antitumor effects of flavanones in vitro and in vivo: involvement of caspase 3 activation p21 gene expression, and reactive oxygen species production." 197 : 84-95, 2004

      10 "Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids" 66 : 1139-1150, 2003

      1 "The role of proteases during apoptosis" 10 : 587-597, 1996

      2 "The role of AP-1, NF-kappaB and ROS/NOS in skin carcinogenesis: the JB6 model is predictive." 234 : 185-193, 2002

      3 "The radioprotective effects of radices herbs" 41 : 105-111, 2001

      4 "The radioprotective effects of Bu- Zhong-Yi-Qi-Tang A prescription of traditional Chinese medicine" 30 : 127-137, 2002

      5 "The radioprotective effect of Kuei-Pi- Tang as a prescription of traditional Chinese medicine in mice" 28 : 698-704, 1999

      6 "The effects of herbs on the radiation-induced apoptosis in intestinal crypt cells" 26 : 27-33, 2001

      7 "The effects of Shi-Quan-Dai-Bu-Tang and its ingredients on the survival of jejunal crypt cells and hematopoietic cells in irradiated mice" 29 : 93-98, 2000

      8 "The control of apoptosis in mammalian cells" 307-309, 1993

      9 "Structurally related antitumor effects of flavanones in vitro and in vivo: involvement of caspase 3 activation p21 gene expression, and reactive oxygen species production." 197 : 84-95, 2004

      10 "Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids" 66 : 1139-1150, 2003

      11 "Radioprotective effects of two traditional Chinese medicine prescriptions: Si-Wu-Tang and Si-Jun-Zi-Tang." 27 : 387-396, 1999

      12 "Protein oxidation in aging, disease, and oxidative stress." 272 : 20313-20316, 1997

      13 "Protective effects of Paeonia japonica against radiation-induced damage" 27 : 181-188, 2002

      14 "Protective effect of a herbal preparation (HemoHIM) on the self-renewal tissues and immune system against γ- irradiation" 34 : 805-813, 2005

      15 "Protection of human endothelial cells from oxidative stress: role of Ras-ERK1/2 signaling." 105 : 968-974, 2002

      16 "Inhibition of lipopolysaccharide-induced nitric oxide production by flavonoids in RAW264.7 macrophages involves hemo oxygenase-1." 66 : 1821-1832, 2003

      17 "Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species" 36 : 897-910, 2004

      18 "Effects of a herb mixture (HIM-I) on the protection of the hematopoietic-immune system and self-renewal tissues against radiation damage" 34 : 605-612, 2005

      19 "Effect of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes" 67 : 1210-1218, 1998

      20 "Effect of Si-Wu-Tang and Si-Jun-Zi-Tang on the survival of jejunal crypt cells and hematopoietic cells in irradiated mice" 30 : 888-894, 1998

      21 "Detection of the initial stages of DNA fragmentation in apoptosis" 1032-1036, 1993

      22 "Cytometry in cell necrobiology: Analysis of apoptosis and accidental cell death (necrosis)." 27 : 1-20, 1997

      23 "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis" 391 : 96-99, 1998

      24 "Chronic oxidative stress compromise telomere integrity and accelerates the onset of senscence in human endothelial cells" 117 : 2417-2426, 2004

      25 "Camptothecin causes cell cycle perturbations within T-lymphoblastoid cells followed by dose dependent induction of apoptosis" 21 : 961-972, 1997

      26 "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." 184 : 39-51, 1995

      27 "3-OH flavone inhibition of epidermal growth factor-induced proliferation through blocking prostaglandin E2 production" 108 : 502-510, 2004

      더보기

      동일학술지(권/호) 다른 논문

      동일학술지 더보기

      더보기

      분석정보

      View

      상세정보조회

      0

      Usage

      원문다운로드

      0

      대출신청

      0

      복사신청

      0

      EDDS신청

      0

      동일 주제 내 활용도 TOP

      더보기

      주제

      연도별 연구동향

      연도별 활용동향

      연관논문

      연구자 네트워크맵

      공동연구자 (7)

      유사연구자 (20) 활용도상위20명

      인용정보 인용지수 설명보기

      학술지 이력

      학술지 이력
      연월일 이력구분 이력상세 등재구분
      2023 평가예정 해외DB학술지평가 신청대상 (해외등재 학술지 평가)
      2020-01-01 평가 등재학술지 유지 (해외등재 학술지 평가) KCI등재
      2014-06-24 학회명변경 한글명 : 한국식품영양과학회지 -> 한국식품영양과학회
      영문명 : Journal of the Korean Society of Food Science and Nutrition -> The Korean Society of Food Science and Nutrition
      KCI등재
      2014-04-02 학회명변경 한글명 : 한국식품영양과학회 -> 한국식품영양과학회지
      영문명 : 미등록 -> Journal of the Korean Society of Food Science and Nutrition
      KCI등재
      2011-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2009-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2007-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2005-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2002-07-01 평가 등재학술지 선정 (등재후보2차) KCI등재
      2000-01-01 평가 등재후보학술지 선정 (신규평가) KCI등재후보
      더보기

      학술지 인용정보

      학술지 인용정보
      기준연도 WOS-KCI 통합IF(2년) KCIF(2년) KCIF(3년)
      2016 1.03 1.03 1.13
      KCIF(4년) KCIF(5년) 중심성지수(3년) 즉시성지수
      1.18 1.2 1.993 0.21
      더보기

      이 자료와 함께 이용한 RISS 자료

      나만을 위한 추천자료

      해외이동버튼