환경 유형에 따른 바이오에어로졸 중 배양성 세균 동정 및 계통분석 = Identification and Phylogenetic Analysis of Culturable Bacteria in the Bioareosol from Several Environments

국문 초록 (Abstract)

바이오에어로졸은 0.02-100 μm 입자로, 자연 및 인위적 환경 또는 사람의 활동에 의해 발생된다. 바이오에어로졸은 바이러스, 세균, 곰팡이, 원생생물, 곰팡이 포자, 미생물 독소, 꽃가루, 동식물 기원의 물질, 타액 또는 글루칸 등으로 구성되며, 인간과 동물에게 호흡기 등의 질환을 유발한다. 본 연구에서는 농업, 축산, 하수처리장, 해변 및 청청지역의 바이오에어로졸 시료에서 분리한 배양성 미생물을 동정 및 계통분석을 수행한 결과, 우점 및 종의 구성 등 환경 유형 별 차이가 분석되었다. 한편 모든 시료에서 31 분리주가 Bacillus cereus로 동정되었고, 실내 양계장에서 Acinetobacter baumannii가 분리되었다. 또한 농업, 축산 및 해변에서 분리한 미생물 중 Domibacillus속, Chryceobacterium속, Nocardioides속 및 Comamonadaceae과에 속하는 새로운 종 또는 속이 분리되었다.

다국어 초록 (Multilingual Abstract)

Bioaerosols are comprised of particles 0.02-100 μm in size that originate in natural and artificial environments, and as a result of human activities. They consist of microorganisms including viruses, bacteria, fungi, and protozoa; fungal spores; microbial toxins; pollen; plant or animal material; expectorated liquid from humans; and glucans (peptidoglycan and β-glucan). Bioaerosols can cause respiratory and other diseases in humans and animals. In this study, bioaerosol samples acquired from agricultural sources, livestock, a sewage treatment plant, a beach, and a pristine area were analyzed to identify and phylogenetically characterize culturable microorganisms. The isolated bacteria exhibited regional differences, with different species dominating. However, Bacillus cereus was isolated in all samples, with a total of 31 strains isolated from all areas, and Acinetobacter baumannii was isolated from an indoor poultry farm. In addition, bacteria determined to be of novel genus or species of the genera Domibacillus, Chryceobacterium, Nocardioides and family Comamonadaceae were isolated from the agricultural, livestock and beach environments.

참고문헌 (Reference)

1 Rho GH, "Studies on the influence of air-born microbes on air pollution" 8 : 27-35, 1983

2 Chun J, "Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences" 78 : 123-127, 2000

3 이시원, "Methylobacterium dankookense sp. nov., Isolated from Drinking Water" 한국미생물학회 47 (47): 716-720, 2009

4 Hong SG, "Mechanical engineering research of the bioaerosol" 53 : 41-44, 2013

5 Tamura K, "MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods" 28 : 2731-2739, 2011

6 Kim OS, "Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species" 62 : 716-721, 2012

7 Robbins CA, "Health effects of mycotoxins in indoor air" 15 : 773-784, 2000

8 Fung F, "Health effects of indoor fungal bioaerosol exposure" 18 : 535-544, 2003

9 Lee S, "Flavobacterium dankookense sp. nov., isolated from a freshwater reservoir and emended descriptions of Flavobacterium cheonanense, F. chungnamense, F. koreense and F. aquatile" 62 : 2378-2382, 2012

10 Kim WH, "Flavihumibacter cheonanensis sp. nov., isolated from sediment of a shallow stream" 65 : 3235-3239, 2014

11 Ko GP, "Exposing of the bioaerosols and risk assessment of human health" 20 : 50-56,

12 Seiler H, "Domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room" 63 : 2054-2061, 2013

13 Hua NP, "Detailed identification of desert-originated bacteria carried by Asian dust storms to Japan" 23 : 291-298, 2007

14 Sharma A, "Description of Domibacillus indicus sp. nov., isolated from ocean sediments and emended description of the genus Domibacillus" 64 : 3010-3015, 2014

15 Jiménez G, "Description of Bacillus toyonensis sp. nov., a novel species of the Bacillus cereus group, and pairwise genome comparisons of the species of the group by means of ANI calculations" 36 : 383-391, 2013

16 남윤형, "Comparison of Chip-Base Real-Time PCR and Tube-Base Real-Time PCR Methods for Detection of B. cereus" 대한화학회 52 (52): 203-206, 2008

17 Leila S, "Comparison of CHROMagarTM acinetobacter and conventional methods for isolation Acinetobacter baumannii from tracheal aspirates patients with ventilator-associated pneumonia (VAP)" 4 : 46-52, 2013

18 김근수, "Comparison of Biofilm Formed on Stainless Steel and Copper Pipe Through the Each Process of Water Treatment Plant" 한국미생물학회 49 (49): 313-320, 2013

19 Hyeon DR, "Chemical composition comparison of fine particulatematter in accordance with meteorological phenomena at background area of Korea during 2012-2013" Jeju National University 2013

20 Lee S, "Characterization of microbial community during Asian dust events in Korea" 407 : 5308-5314, 2009

21 Xu Z, "Bioaerosol science, technology, and engineering: past, present, and future" 45 : 1337-1349, 2011

22 Douwes J, "Bioaerosol health effects and exposure assessment: progress and prospects" 47 : 187-200, 2003

23 Gatson JW, "Bacillus tequilensis sp. nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis" 56 : 1475-1484, 2006

24 Shivaji S, "Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes" 56 : 1465-1473, 2006

25 Steffen E, "Air sampling of Aspergillus fumigatus and other thermotolerant fungi: Comparative performance of the Sartorius MD8 airport and the Merck MAS-100 portable bioaerosol sampler" 210 : 733-739, 2006

26 Kellogg CA, "Aerobiology and the global transport of desert dust" 21 : 638-644, 2006