돼지 설사병은 부종병과 함께 양돈 농가에 막대한 경제적 손실을 입히는 가축질병으로 알려져 있으며, 병원성 대장균의 증식에 의하여 설사 및 대장균 생성 독소에 의한 부종병이 동반된다....
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https://www.riss.kr/link?id=A107837383
2021
Korean
KCI등재
학술저널
755-760(6쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
돼지 설사병은 부종병과 함께 양돈 농가에 막대한 경제적 손실을 입히는 가축질병으로 알려져 있으며, 병원성 대장균의 증식에 의하여 설사 및 대장균 생성 독소에 의한 부종병이 동반된다....
돼지 설사병은 부종병과 함께 양돈 농가에 막대한 경제적 손실을 입히는 가축질병으로 알려져 있으며, 병원성 대장균의 증식에 의하여 설사 및 대장균 생성 독소에 의한 부종병이 동반된다. 본 연구에서는 항균펩타이드를 포함하는 면역유도누에의 조추출물을 이용하여 돼지 설사 및 부종을 유발하는 F4+ 및 F18ab+ 대장균의 사균체를 제작하고 사균백신으로서 이용가능성을 검토하였다. 면역유도누에 추출물의 항균활성을 환확산법을 통해 분석한 결과 F4+ 및 F18ab+ 대장균에 대하여 높은 활성을 나타내었으며, 평판계수법을 이용하여 대장균의 사균체 생산 효율을 분석한 결과 50 ㎎/ml의 농도를 4℃에서 처리했을 때 가장 높게 나타났다. 또한 제작된 사균체에 대해서 투과전자현미경을 이용하여 분석한 결과 세포질은 유출되고 세포막만이 남아있는 대장균이 확인되었다. 따라서 면역유도누에 추출물을 이용하여 제작된 F4+ 및 F18ab+ 대장균은 부종병 예방을 위한 사균백신으로서 이용가능성이 매우 높을 것으로 판단된다.
다국어 초록 (Multilingual Abstract)
Swine diarrhea is a livestock disease that causes huge economic losses to pig farms. In general, diarrhea occurs because of the proliferation of pathogenic Escherichia coli (E. coli). The toxins produced by the proliferated E. coli cause edema in pigs...
Swine diarrhea is a livestock disease that causes huge economic losses to pig farms. In general, diarrhea occurs because of the proliferation of pathogenic Escherichia coli (E. coli). The toxins produced by the proliferated E. coli cause edema in pigs. Although the proliferation of these coliforms can be prevented by using a vaccine, the vaccines containing chemically produced dead bacteria are not very effective, making it difficult to control the proliferation of E. coli. Therefore, there is a need to develop new, more effective vaccines. In this study, we prepared killed F4+ and F18ab+ E. coli, which induce diarrhea and edema in pigs, using the extracts of immune-induced silkworms containing antimicrobial peptides and examined their availability as a killed-bacteria vaccine. First, the antimicrobial activity analysis of the prepared immune-induced silkworm extract was conducted using the radial diffusion assay. The results showed high activity against both F4+ and F18ab+ E. coli. The production efficiency of E. coli dead cells was determined using the colony-counting method. The concentration of the E. coli dead cells was the highest (50 ㎎/ml) when treated at 4℃. In addition, the analysis of the prepared dead cells using a transmission electron microscope confirmed that E. coli leaked out of the cytoplasm and the cell membrane remained intact. Therefore, F4+ and F18ab+ E. coli produced using immune-induced silkworms extract are considered to be highly available as bacterial ghost vaccines that can help prevent swine diarrhea and the resulting edema.
참고문헌 (Reference)
1 오륜경, "참담치(Mytilus coruscus) 유래 항균 펩타이드 분리 및 정제" 한국생명과학회 26 (26): 1259-1268, 2016
2 Buzby, J. C., "The economics of enteric infections : human foodborne disease costs" 136 : 1851-1862, 2009
3 Lei, J., "The antimicrobial peptides and their potential clinical applications" 11 : 3919-3931, 2019
4 Xu, G., "Prevalence and characteristics of extended-spectrum beta-lactamase genes in Escherichia coli isolated from piglets with postweaning diarrhea in Heilongjiang province, China" 6 : 1103-, 2015
5 지상덕, "Nutrition composition differences among steamed and freeze-dried mature silkworm larval powders made from 3 Bombyx mori varieties weaving different colored cocoons" 한국잠사학회 33 (33): 6-14, 2016
6 Raheem, N., "Mechanisms of action for antimicrobial peptides with antibacterial and antibiofilm functions" 10 : 2866-, 2019
7 Kim, S. R., "Isolation and purification of a cecropin-like antimicrobial peptide from the Japanese oak silkworm, Antheraea yamamai" 50 : 145-149, 2012
8 Fairbrother, J. M., "Immunogenicity and protective efficacy of a single-dose live non-pathogenic Escherichia coli oral vaccine against F4-positive enterotoxigenic Escherichia coli challenge in pigs" 35 : 353-360, 2017
9 Yamakawa, M., "Immune proteins and their gene expression in the silkworm, Bombyx mori" 23 : 281-289, 1999
10 Nguyen, U. V., "High susceptibility prevalence for F4(+)and F18(+)Escherichia coli in Flemish pigs" 202 : 52-57, 2017
1 오륜경, "참담치(Mytilus coruscus) 유래 항균 펩타이드 분리 및 정제" 한국생명과학회 26 (26): 1259-1268, 2016
2 Buzby, J. C., "The economics of enteric infections : human foodborne disease costs" 136 : 1851-1862, 2009
3 Lei, J., "The antimicrobial peptides and their potential clinical applications" 11 : 3919-3931, 2019
4 Xu, G., "Prevalence and characteristics of extended-spectrum beta-lactamase genes in Escherichia coli isolated from piglets with postweaning diarrhea in Heilongjiang province, China" 6 : 1103-, 2015
5 지상덕, "Nutrition composition differences among steamed and freeze-dried mature silkworm larval powders made from 3 Bombyx mori varieties weaving different colored cocoons" 한국잠사학회 33 (33): 6-14, 2016
6 Raheem, N., "Mechanisms of action for antimicrobial peptides with antibacterial and antibiofilm functions" 10 : 2866-, 2019
7 Kim, S. R., "Isolation and purification of a cecropin-like antimicrobial peptide from the Japanese oak silkworm, Antheraea yamamai" 50 : 145-149, 2012
8 Fairbrother, J. M., "Immunogenicity and protective efficacy of a single-dose live non-pathogenic Escherichia coli oral vaccine against F4-positive enterotoxigenic Escherichia coli challenge in pigs" 35 : 353-360, 2017
9 Yamakawa, M., "Immune proteins and their gene expression in the silkworm, Bombyx mori" 23 : 281-289, 1999
10 Nguyen, U. V., "High susceptibility prevalence for F4(+)and F18(+)Escherichia coli in Flemish pigs" 202 : 52-57, 2017
11 Brisse, M., "Emerging concepts and technologies in vaccine development" 11 : 2578-, 2020
12 Nadeau, E., "Efficacy of a single oral dose of a live bivalent E. coli vaccine against post-weaning diarrhea due to F4 and F18-positive enterotoxigenic E. coli" 226 : 32-39, 2017
13 Zhu, C., "Development of live attenuated bacterial vaccines targeting Escherichia coli heat-labile and heat-stable enterotoxins" 202 : 72-78, 2017
14 Steinberg, D. A., "Designer assays for antimicrobial peptides" 78 : 169-186, 1997
15 Itoh, Y., "Depolymerization of beta-1, 6-N-acetyl-Dglucosamine disrupts the integrity of diverse bacterial biofilms" 187 : 382-387, 2005
16 Szostak, M. P., "Bacterial ghosts : non-living candidate vaccines" 44 : 161-170, 1996
17 Lv, Y., "Antimicrobial properties and membrane-active mechanism of a potential alpha-helical antimicrobial derived from cathelicidin PMAP-36" 9 : e86364-, 2014
18 Li, Z., "Antimicrobial peptides in silkworm" 69 : 391-410, 2019
19 Montesinos, E., "Antimicrobial peptides and plant disease control" 270 : 1-11, 2007
20 Hancock, R. E., "Antimicrobial and hostdefense peptides as new anti-infective therapeutic strategies" 24 : 1551-1557, 2006
21 Ishii, K., "Activation of the silkworm cytokine by bacterial and fungal cell wall components vis a reactive oxygen species-triggered mechanism" 283 : 2185-2191, 2008
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2027 | 평가예정 | 재인증평가 신청대상 (재인증) | |
2021-01-01 | 평가 | 등재학술지 유지 (재인증) | |
2018-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2015-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2011-08-03 | 학술지명변경 | 외국어명 : Korean Journal of Life Science -> Journal of Life Science | |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2003-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2001-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.37 | 0.37 | 0.42 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.43 | 0.43 | 0.774 | 0.09 |