Korean-radish peroxidase can replace horseradish peroxidase for enzymatic determination of glucose. The glucose could be quantitatively assayed by using glucose oxidase and Korean-radish peroxidase. The optimum temperature and pH for glucose assay wer...
Korean-radish peroxidase can replace horseradish peroxidase for enzymatic determination of glucose. The glucose could be quantitatively assayed by using glucose oxidase and Korean-radish peroxidase. The optimum temperature and pH for glucose assay were of 40℃ and pH 5.5, respectively. Various cations and anions such as Ag^+, Cu^(++), Pb^(++), Fe^(++), N₃^-, S₂O₃^-, S₂O₄^- and NO₂^- inhibited the glucose assay completely. The relationship between absorbance and glucose concentration was linear up to 50μ㏖ of glucose under the optimum assay conditions.