미세유체시스템을 이용한 효소 함유 하이드로젤 기반 알코올 분석 바이오센서 개발 = Development of enzyme-containing PEG hydrogel microarray within microfluidic device for ethanol detection

다국어 초록 (Multilingual Abstract)

This paper describes a simple method to fabricate a microfluidic biosensor that can optically detect alcohol. This system is composed of microchannels connected with six inlet and outlet, which have width and height approximately 100um and 30um, respectively. The oxidase enzymes were entrapped with the poly(ethyleneglycol)(PEG)-based hydrogel microstructure that was fabricated within the microchannels by a photolithography process. Alcohol oxidase(AOX) and peroxidase(POD) were chosen as the model enzyme which resulted in a fluorescent hydrogel microarray that was responsive to alcohol. The hydrogel-entrapped AOX could perform enzyme-catalyzed oxidase of alcohol to produce H2O2 which reacted with POD to produce highly fluorescent resorufin. The fluorescence intensity of the hydrogel microstructures increased as the alcohol concentrations increased, and alcohol detection was possible in the concentration range from 1.0 to 10mM using fluorescence indicator in a single detection chamber.

참고문헌 (Reference)

1 P. Yager, "Microfluidic diagnostic technologies for global public health" 442 (442): 412-, 2006

2 E. Jang, "Microfluidic bioassay system based on microarrays of hydrogel sensing elements entrapping quantum dot–enzyme conjugates" 31 (31): 529-, 2011

3 R. P. Huang, "Detection of multiple proteins in an antibody-based protein microarray system" 255 (255): 1-, 2001

4 R. Benters, "Dendrimer-Activated Solid Supports for Nucleic Acid and Protein Microarrays" 2 (2): 686-, 2001

5 A. J. deMello, "Control and detection of chemical reactions in microfluidic system" 442 (442): 394-, 2006

6 D. A. Zubtsov, "Comparison of surface and hydrogel-based protein microchips" 368 (368): 205-, 2006

7 J. El-Ali, "Cell on a chip" 442 (442): 403-, 2006