Toxin Gene Profiling of Bacillus cereus Food Isolates by PCR

다국어 초록 (Multilingual Abstract)

Seventy-one Bacillus cereus strains (12 references and 59 food isolates) were analyzed for the
occurrence of five different enterotoxin genes (nheABC, hblCDA, entFM, cytK, and bceT) and one
emetic toxin cereulide synthetase gene (ces) by PCR (polymerase chain reaction). PCR analysis
revealed eight toxigenic patterns in all B. cereus strains tested; they all carried both entFM and
nheABC. The presence of hblCDA, cytK, and bceT varied according to the enterotoxin-producing
strains, among which hblCDA was the least frequently detected in the food-isolated strains. Only
five B. cereus strains harbored ces, associated with the emetic type of food poisoning; however,
these strains were devoid of hblCDA, cytK, and bceT.

다국어 초록 (Multilingual Abstract)

Seventy-one Bacillus cereus strains (12 references and 59 food isolates) were analyzed for the
occurrence of five different enterotoxin genes (nheABC, hblCDA, entFM, cytK, and bceT) and one
emetic toxin cereulide synthetase gene (ces) by PCR (polymerase chain reaction). PCR analysis
revealed eight toxigenic patterns in all B. cereus strains tested; they all carried both entFM and
nheABC. The presence of hblCDA, cytK, and bceT varied according to the enterotoxin-producing
strains, among which hblCDA was the least frequently detected in the food-isolated strains. Only
five B. cereus strains harbored ces, associated with the emetic type of food poisoning; however,
these strains were devoid of hblCDA, cytK, and bceT.

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