Improved Isopropanol Productivity by Using Lipid Metabolic Pathway in Oleaginous Yeast Yarrowia lipolytica

다국어 초록 (Multilingual Abstract)

Isopropanol is converted industrially to propylene, which is important not only as a fuel but also as a backbone chemical for bioplastics. One of high stress tolerant yeasts, Yarrowia lipolytica is an oleaginous strain possessing relatively high native acetyl-CoA levels. Here, we genetically engineered a Y. lipolytica strain, which is expected to increase the yield of isopropanol. Malonyl-CoA is a major substrate exhibiting a high synthesis rate and high concentration in a lipid metabolic pathway. When the acetoacetyl-CoA synthase gene (nphT7) synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA is used, isopropanol productivity can be expected to increase the yield of isopropanol. This experiment showed that 300 ㎎/L of isopropanol was produced under the shaking flasks cultivation condition, representing two-fold increase in isopropanol titer compared to previous studies. Taken together, this is the first report of the isopropanol production by using lipid metabolic pathway in Y. lipolytica.