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      Trametes villosa Lignin Peroxidase (TvLiP): Genetic and Molecular Characterization = Trametes villosa Lignin Peroxidase (TvLiP): Genetic and Molecular Characterization

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      https://www.riss.kr/link?id=A102717001

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      다국어 초록 (Multilingual Abstract)

      White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the 7<sup>th</sup> day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.
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      White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regardi...

      White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the 7<sup>th</sup> day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.

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      참고문헌 (Reference)

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      2 Chen M, "Understanding lignin-degrading reactions of ligninolytic enzymes: binding affinity and interactional profile" 6 : 25647-, 2011

      3 Brown NA, "The predicted secretome of the plant pathogenic fungus Fusarium graminearum: a refined comparative analysis" 7 : e33731-, 2012

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      1 Pérez-Boada M, "Versatile peroxidase oxidation of high redox potential aromatic compounds: sitedirected mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways" 354 : 385-402, 2005

      2 Chen M, "Understanding lignin-degrading reactions of ligninolytic enzymes: binding affinity and interactional profile" 6 : 25647-, 2011

      3 Brown NA, "The predicted secretome of the plant pathogenic fungus Fusarium graminearum: a refined comparative analysis" 7 : e33731-, 2012

      4 Floudas D, "The Paleozoic origin of enzymatic lignin decomposition reconstructed from 31 fungal genomes" 336 : 1715-1719, 2012

      5 Cantarel BL, "The Carbohydrate-Active EnZymes database (CAZy): an expert resource for glycogenomics" 37 : 233-238, 2009

      6 Ospina-Giraldo MD, "The CAZyome of Phytophthora spp: a comprehensive analysis of the gene complement coding for carbohydrateactive enzymes in species of the genus Phytophthora" 11 : 525-, 2010

      7 Ruiz-Dueñas F, "Substrate oxidation sites in versatile peroxidase and other basidiomycete peroxidases" 60 : 441-452, 2009

      8 Wong DWS., "Structure and action mechanism of ligninolytic enzymes" 157 : 174-209, 2009

      9 Koyani RD, "Solid state fermentation:comprehensive tool for utilization of lignocellulosic through biotechnology" 5 : 2-, 2015

      10 Kuwahara M, "Separation and characterization of two extracellular H2O2dependent oxidases from lignolytic cultures of Phanerochaete chrysosporium" 169 : 247-250, 1984

      11 Hammel K, "Role of fungal peroxidases in biological ligninolysis" 11 : 349-355, 2008

      12 Matheny PB, "Resolving the phylogenetic position of the Wallemiomycetes: an enigmatic major lineage of Basidiomycota" 84 : 1794-1805, 2006

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      15 Silva MLC, "Production of manganese peroxidase by Trametes villosa on inexpensive substrate and its application in the removal of lignin from agricultural wastes" 5 : 1067-1077, 2014

      16 Guerra G, "Production of laccase and manganese peroxidase by the white-rot fungi from sugarcane bagasse in solid bed use for dyes decolourisation" 10 : 260-264, 2008

      17 Knežević A, "Potential of Trametes species to degrade lignin" 85 : 52-56, 2013

      18 Iqbal HMN, "Optimization of physical and nutritional factors for synthesis of lignin degrading enzymes by a novel strain of Trametes versicolor" 6 : 1273-1278, 2011

      19 Morgenstern I, "Molecular evaluation and diversity of lignin degrading heme peroxidase in the Agaricomycetes" 66 : 243-257, 2008

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      21 Wariishi H, "Manganese(II)oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators" 267 : 23688-23695, 1992

      22 Yamanaka R, "Lignolytic enzymes produced by Trametes villosa CCB176under different culture conditions" 39 : 78-84, 2008

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      24 Tien M, "Lignin-degrading enzyme from Phanerochaete chrysosporium: purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase" 81 : 2280-2284, 1984

      25 Morozova OV, "Laccase-mediator system and their applications: a review" 43 : 523-535, 2007

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      32 Kües U., "Fungal enzymes for environmental management" 33 : 268-278, 2015

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      38 Rakrudee Sarnthima, "Extracellular Ligninolytic Enzymes by Lentinus polychrous Lév. under Solid-state Fermentation of Potential Agro-industrial Wastes and Their Effectiveness in Decolorization of Synthetic Dyes" 한국생물공학회 14 (14): 513-522, 2009

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      40 Levasseur A, "Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes" 6 : 41-, 2013

      41 Quiroz-Castañeda RE, "Evaluation of different lignocellulosic substrates for the production of cellulases and xylanases by the basidiomycete fungi Bjerkandera adusta and Pycnoporus sanguineus" 22 : 565-572, 2011

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      43 Moredo N, "Enhanced ligninolytic enzyme production and degrading capability of Phanerochaete chrysosporium and Trametes versicolor" 19 : 665-669, 2003

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      45 Elisashvili V, "Effect of growth substrate, method of fermentation, and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes" 35 : 1531-1538, 2008

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      48 Góes-Neto A, "DNA extraction from frozen field-collected and dehydrated herbarium fungal basidiomata: performance of SDS and CTAB-based methods" 18 : 19-32, 2005

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      50 Fitch WM, "Construction of phylogenetic trees" 155 : 279-284, 1967

      51 Zhao Z, "Comparative analysis of fungal genomes reveals different plant cell wall degrading capacity in fungi" 15 : 6-, 2014

      52 Galhaup C, "Characterization of the major laccase isoenzyme from Trametes pubescens and regulation of its synthesis by metal ions" 148 : 2159-2169, 2002

      53 Singh AP, "Biotechnological applications of wood-rotting fungi: a review" 62 : 198-206, 2014

      54 Silva R, "Aplicações de fibras lignocelulósicas na Química de polímeros e em compósitos" 32 : 661-671, 2009

      55 Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method" 25 : 402-408, 2001

      56 Tadesse MA, "An assessment of the relative contributions of redox and steric issues to laccase specificity towards putative substrates" 6 : 868-878, 2008

      57 Singh R, "A review on delignification of lignocellulosic biomass for enhancement of ethanol production potential" 32 : 713-728, 2014

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      59 Johasson T, "A cluster of genes encoding major isozymes of lignin peroxidase, and manganese peroxidase from the white-rot fungus Trametes versicolor" 170 : 31-38, 1996

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      학술지 이력

      학술지 이력
      연월일 이력구분 이력상세 등재구분
      2023 평가예정 해외DB학술지평가 신청대상 (해외등재 학술지 평가)
      2020-01-01 평가 등재학술지 유지 (해외등재 학술지 평가) KCI등재
      2010-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2008-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2006-04-04 학술지명변경 한글명 : -> Journal of Microbiology and Biotechnology KCI등재
      2006-03-30 학술지등록 한글명 :
      외국어명 : Journal of Microbiology and Biotechnology
      KCI등재
      2006-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2004-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2001-07-01 평가 등재학술지 선정 (등재후보2차) KCI등재
      1999-01-01 평가 등재후보학술지 선정 (신규평가) KCI등재후보
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      학술지 인용정보
      기준연도 WOS-KCI 통합IF(2년) KCIF(2년) KCIF(3년)
      2016 1.59 0.33 1.17
      KCIF(4년) KCIF(5년) 중심성지수(3년) 즉시성지수
      0.91 0.78 0.472 0.08
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