Glucoraphanin can be hydrolyzed into sulforaphane with biological activity under the action of myrosinase. Sulforaphane has strong anti-cancer activity, however, the effect and the mechanism of anti-obesity activity are not yet clear. In this study, w...
Glucoraphanin can be hydrolyzed into sulforaphane with biological activity under the action of myrosinase. Sulforaphane has strong anti-cancer activity, however, the effect and the mechanism of anti-obesity activity are not yet clear. In this study, we evaluated the anti-obesity effects of broccoli sprouts powder rich in glucoraphanin, mustard seed powder with high activity myrosinase, and mustard and broccoli mixture powder (MBP) on 3T3-L1 adipocytes, respectively. The results showed that MBP can significantly inhibited the expression of adipogenic genes and increased the expression of genes related to lipolysis and lipid metabolism. MBP inhibits the protein expression of adipogenic transcription factor peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein (C/EBPα), and fatty acid-binding protein (aP2) in 3T3-L1 adipocytes, and inhibits the protein expression of fatty acid synthase gene protein through AMP-activated protein kinase. Which also increases the expression of carnitine palmitoyltransferase-1 (CPT-1) lipolysis-related genes. These results suggest that MBP may have anti-obesity effects on adipogenesis and lipid metabolism.