RISS 검색 - 국내학술지논문 상세보기

다국어 초록 (Multilingual Abstract)

Background: Soil microorganisms play key roles in nutrient cycling and are distributed throughout the soil profile.
Currently, there is little information about the characteristics of the microbial communities along the soil depth because most studies focus on microorganisms inhabiting the soil surface. To better understand the functions and composition of microbial communities and the biogeochemical factors that shape them at different soil depths, we analyzed microbial activities and bacterial and fungal community composition in soils up to a 120 cm depth at a fallow field located in central Korea. To examine the vertical difference of microbial activities and community composition, β-1,4-glucosidase, cellobiohydrolase, β-1,4-xylosidase, β-1,4-N-acetylglucosaminidase, and acid phosphatase activities were analyzed and barcoded pyrosequencing of 16S rRNA genes (bacteria) and internal transcribed spacer region (fungi) was conducted.
Results: The activity of all the soil enzymes analyzed, along with soil C concentration, declined with soil depth. For example, acid phosphatase activity was 125.9 (± 5.7 (± 1 SE)), 30.9 (± 0.9), 15.7 (± 0.6), 6.7 (± 0.9), and 3.3 (± 0.3) nmol g−1 h−1 at 0–15, 15–30, 30–60, 60–90, and 90–120 cm soil depths, respectively. Among the bacterial groups, the abundance of Proteobacteria (38.5, 23.2, 23.3, 26.1, and 17.5% at 0–15, 15–30, 30–60, 60–90, and 90–120 cm soil depths, respectively) and Firmicutes (12.8, 11.3, 8.6, 4.3, and 0.4% at 0–15, 15–30, 30–60, 60–90, and 90–120 cm soil depths, respectively) decreased with soil depth. On the other hand, the abundance of Ascomycota (51.2, 48.6, 65.7, 46.1, and 45.7% at 15, 30, 60, 90, and 120 cm depths, respectively), a dominant fungal group at this site, showed no clear trend along the soil profile.
Conclusions: Our results show that soil C availability can determine soil enzyme activity at different soil depths and that bacterial communities have a clear trend along the soil depth at this study site. These metagenomics studies, along with other studies on microbial functions, are expected to enhance our understanding on the complexity of soil microbial communities and their relationship with biogeochemical factors.

참고문헌 (Reference)

1 Fierer, N., "Variations in microbial community composition through two soil depth profiles" 35 : 167-176, 2003

2 Fierer, N., "Toward an ecological classification of soil bacteria" 88 : 1354-1364, 2007

3 Saiya-Cork, K. R., "The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil" 34 : 1309-1315, 2002

4 Buss, H. L., "The coupling of biological iron cycling and mineral weathering during saprolite formation, Luquillo Mountains, Puerto Rico" 3 : 247-260, 2005

5 Chun, J., "The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454GS FLX titanium pyrosequencer" 10 : 101-, 2010

6 Blume, E., "Surface and subsurface microbial biomass, community structure and metabolic activity as a function of soil depth and season" 20 : 171-181, 2002

7 Axelrood, P. E., "Molecular characterization of bacterial diversity from British Columbia forest soils subjected to disturbance" 48 : 655-674, 2002

8 McCaig, A. E., "Molecular analysis of bacterial community structure and diversity in unimproved and improved upland grass pastures" 65 : 1721-1730, 1999

9 Nannipieri, P., "Microbial diversity and soil functions" 54 : 655-670, 2003

10 Goberna, M., "Microbial community structure at different depths in disturbed and undisturbed semiarid mediterranean forest soils" 50 : 315-326, 2005