Modulation of Inula racemosa Hook Extract on Cardioprotection by Ischemic Preconditioning in Hyperlipidaemic Rats

다국어 초록 (Multilingual Abstract)

Objectives: Hyperlipidemia (HL) is a major cause of ischemic heart diseases. The size-limiting effect of ischemic preconditioning (IPC), a cardioprotective phenomenon, is reduced in HL, possibly because of the opening of the mitochondrial permeability transition pore (MPTP). The objective of this study is to see what effect pretreatment with Inula racemosa Hook root extract (IrA) had on IPC-mediated cardioprotection on HL Wistar rat hearts. An isolated rat heart was mounted on the Langendorff heart array, and then ischemia reperfusion (I/R) and IPC cycles were performed. Atractyloside (Atr) is an MPTP opener.
Methods: The animals were divided into ten groups, each consisting of six rats (n = 6), to investigate the modulation of I. racemosa Hook extract on cardioprotection by IPC in HL hearts: Sham control, I/R Control, IPC control, I/R + HL, I/R + IrA + HL, IPC + HL, IPC + NS + HL, IPC + IrA+ HL, IPC + Atr + oxidative stress, mitochondrial function, integrity, and hemodynamic parameters are evaluated for each group.
Results: The present experimental data show that pretreatment with IrA reduced the LDH, CK-MB, size of myocardial infarction, content of cardiac collagen, and ventricular fibrillation in all groups of HL rat hearts. This pretreatment also reduced the oxidative stress and mitochondrial dysfunction. Inhibition of MPTP opening by Atr diminished the effect of IrA on IPC-mediated cardioprotection in HL rats.
Conclusion: The study findings indicate that pretreatment with IrA e restores IPC-mediated cardioprotection in HL rats by inhibiting the MPTP opening.

참고문헌 (Reference)

1 Sharma S, "The global burden of cardiovascular disease in women" 20 (20): 81-, 2018

2 Vatner DE, "Secreted frizzled-related protein 2, a novel mechanism to induce myocardial ischemic protection through angiogenesis" 115 (115): 48-, 2020

3 Rohilla R, "Role of heme oxygenase- 1(HO-1) and endothelin-1 (ET-1) in modulation of cardioprotective effect of ischemic postconditioning in diabetic rat heart" 54 (54): 690-697, 2020

4 Varshney V, "Role of erythropoietin in ischemic postconditioning induced cardioprotection in hyperlipidemic rat heart" 7 (7): 72-77, 2017

5 Charan K, "Role of atrial natriuretic peptide in ischemic preconditioning-induced cardioprotection in the diabetic rat heart" 201 (201): 272-278, 2016

6 Bell RM, "Retrograde heart perfusion : the Langendorfftechnique of isolated heart perfusion" 50 (50): 940-950, 2011

7 Shabab S, "Protective effects of medicinal plant against diabetes induced cardiac disorder : a review" 265 : 113328-, 2021

8 Pedersen PL, "Preparation and characterization of mitochondria and submitochondrial particles of rat liver and liverderived tissues" 20 : 411-481, 1978

9 Kalachaveedu M, "Phytoestrogenic effect of Inula racemosa Hook f-a cardioprotective root drug in traditional medicine" 210 : 408-416, 2018

10 Mohan S, "Phytochemical analysis and differential in vitro cytotoxicity assessment of root extracts of Inula racemosa" 89 : 781-795, 2017

11 Yadav HN, "Modulation of the cardioprotective effect of ischemic preconditioning in hyperlipidaemic rat heart" 643 (643): 78-83, 2010

12 García-Niño WR, "Mitochondrial quality control in cardiac-conditioning strategies against ischemia-reperfusion injury" 11 (11): 1123-, 2021

13 Oldenburg O, "Mitochondrial K(ATP) channels: role in cardioprotection" 55 (55): 429-437, 2002

14 Hosseini L, "Melatonin and nicotinamide mononucleotide attenuate myocardial ischemia/reperfusion injury via modulation of mitochondrial function and hemodynamic parameters in aged rats" 25 (25): 240-250, 2020

15 Garg K, "Mechanism of cardioprotective effect of erythropoietin-induced preconditioning in rat heart" 42 (42): 219-223, 2010

16 Javadov SA, "Ischaemic preconditioning inhibits opening of mitochondrial permeability transition pores in the reperfused rat heart" 549 (549): 513-524, 2003

17 Vishwakarma VK, "Involvement of atrial natriuretic peptide in abrogated cardioprotective effect of ischemic preconditioning in ovariectomized rat heart" 37 (37): 704-713, 2018

18 Yadav HN, "Involvement of GSK-3β in attenuation of the cardioprotective effect of ischemic preconditioning in diabetic rat heart" 343 (343): 75-81, 2010

19 Ferdinandy P, "Interaction of cardiovascular risk factors with myocardial ischemia/reperfusion injury, preconditioning, and postconditioning" 59 (59): 418-458, 2007

20 Hausenloy DJ, "Inhibiting mitochondrial permeability transition pore opening: a new paradigm for myocardial preconditioning?" 55 (55): 534-543, 2002

21 Buelna-Chontal M, "Implications of oxidative and nitrosative post-translational modifications in therapeutic strategies against reperfusion damage" 10 (10): 749-, 2021

22 William N, "High sub-zero organ preservation : a paradigm of nature-inspired strategies" 102 : 15-26, 2021

23 Wang Y, "Functionalized nanoparticles with monocyte membranes and rapamycin achieve synergistic chemoimmunotherapy for reperfusioninduced injury in ischemic stroke" 19 (19): 331-, 2021

24 Abete P, "Exercise training restores ischemic preconditioning in the aging heart" 36 (36): 643-650, 2000

25 Srivastav RK, "Evaluation of cardioprotective effect of silk cocoon (Abresham) on isoprenaline-induced myocardial infarction in rats" 3 (3): 216-223, 2013

26 Yang X, "Effect of testosterone on the proliferation and collagen synthesis of cardiac fibroblasts induced by angiotensin II in neonatal rat" 8 (8): 14-20, 2017

27 Tiwari AK, "Effect of pravastatin on abrogated cardioprotective effect of late phase of preconditioning in hyperlipidaemic rats" 38 (38): 1645-1653, 2019

28 Penna C, "Effect of hyperglycaemia and diabetes on acute myocardial ischaemia-reperfusion injury and cardioprotection by ischaemic conditioning protocols" 177 (177): 5312-5335, 2020

29 Casin KM, "Dynamic regulation of cysteine oxidation and phosphorylation in myocardial ischemia-reperfusion injury" 10 (10): 2388-, 2021

30 Okada Y, "Cell death induction and protection by activation of ubiquitously expressed anion/cation channels. Part 1: roles of VSOR/VRAC in cell volume regulation, release of double-edged signals and apoptotic/necrotic cell death" 8 : 614040-, 2021

31 Lokhande PD, "Cardiac activity of isolated constituents of Inula racemosa" 6 (6): 81-88, 2006

32 Pachauri P, "Angiotensin (1-7) facilitates cardioprotection of ischemic preconditioning on ischemia-reperfusion-challenged rat heart" 430 (430): 99-113, 2017

33 Ruiz-Meana M, "Ageing, sex, and cardioprotection" 177 (177): 5270-5286, 2020