Assessment of anatomical characteristics of the medicinal plant African cherry (Prunus africana) for its accurate taxonomic identification

다국어 초록 (Multilingual Abstract)

The genus Prunus (family: Rosaceae) consists of over 400 plant species and exhibits vast biodiversity worldwide. Given the wide distribution of this genus, its taxonomic classification is important. Anatomical characteristics are conserved and stable and can therefore be used as an important tool for the taxonomic characterization of plants.
Therefore, this study aimed to assess and document the anatomical characteristics of the leaf, stem, and seed of P. africana using micrographs and photographs for possible use in the identification, quality control, and phylogenetic analysis of the species. The anatomical sections of a young stem revealed a cortex consisting of isodiametric parenchyma cells, druse crystals, primary vascular bundles, and pith. The mature stem bark majorly consisted of the rhytidome, with the periderm densely arranged in multiple layers; a cluster of stone cells; and sclerenchyma. The leaf sections were hypostomatic, with stomata sizes ranging from 18.90- (22.34)-26.90 × 15.41-(18.40)-21.22 μm. The leaf sections showed the presence of characteristic druse crystals, vascular bundles, and mesophyll layers. The pericarp contained the epicarp, mesocarp, and endocarp, with their thickness being approximately 350-400, 300-350, and 30-50 μm, respectively.
In addition, it contained a seed testa with a thickness of approximately 50-60 μm. The morphological and anatomical characteristics observed in P. africana leaves, stems, and seeds in this study could serve as useful data for the taxonomic identification of this species.

참고문헌 (Reference)

1 Jimu L, "Threats and conservation strategies for the African Cherry (Prunus africana) in its natural range-A review" 3 : 118-130, 2011

2 Bastin ES, "Structure of our cherry barks" 67 : 435-453, 1895

3 Abdulrahaman A, "Stomatal complex types, stomatal density, and the stomatal index in some species of Dioscorea" 61 : 2009

4 Vaughan JG, "Seed anatomy and taxonomy" 179 : 251-255, 1968

5 Biswajit D, "Prunus diversity-early and present development: A review" 3 : 721-734, 2011

6 Crang R, "Plant Anatomy" 279-318, 2018

7 Steenkamp V, "Phytomedicines for prostate" 74 : 545-552, 2003

8 Konyar TS, "Occurrence, types and distribution of calcium oxalate crystals in leaves and stems of some species of poisonous plants" 55 : 2014

9 Araújo SJ, "Leaf anatomy as an additional taxonomy tool for 16 species of Malpighiaceae found in the Cerrado area (Brazil)" 286 : 117-131, 2010

10 Kolb LK, "Leaf anatomy as a taxonomy tool for the identification of Brazilian native species of Chionanthus (Oleaceae)" 266 : 151-590, 2020

11 Vincenzo V, "Leaf Morphology, Taxonomy and Geometric Morphometrics: A Simplified Protocol for Beginners" 6 : e25630-, 2011

12 Yeung EC, "Glycol methacrylate: the art of embedding and serial sectioning" 93 : 1-8, 2015

13 Komakech R, "Ethnopharmacological Potential of African Cherry [Prunus africana]" 2019

14 Evert RF, "Esau’s Plant Anatomy" Wiley-Interscience 601-, 2006

15 Lersten RN, "Crystal macropattern development in Prunus serotina (Rosaceae, Prunoideae) Leaves" 97 : 723-729, 2006

16 Hong T, "Characteristics and correlations of leaf stomata in different Aleurites montana provenances" 2018

17 Franceschi RV, "Calcium oxalate in plants: Formation and function" 56 : 41-71, 2005

18 Ilarslan H, "Calcium oxalate crystals in developing seeds of soybean" 88 : 243-257, 2001

19 Lersten RN, "Calcium oxalate crystal macropattern development during Prunus virginiana (Rosaceae) leaf growth" 82 : 2004

20 Mukherjee A, "Botanical identification of medicinal plants: A biosystematics contemplation" 9 : 076-083, 2014

21 Kotina EL, "Bark and wood structure of Prunus africana (Rosaceae), an important African medicinal plant" 106 : 89-95, 2016

22 Wilkinson HP, "Anatomy of the cotyledons 1" Clarendon Press 97-165, 1979

23 De Souza FMD, "Anatomical, phytochemical and histochemical study of Solidago chilensis Meyen" 90 : 2107-2120, 2018

24 Komakech R, "A Review of the Potential of Phytochemicals from Prunus africana (Hook f.) Kalkman Stem Bark for Chemoprevention and Chemotherapy of Prostate Cancer" 0 : 1155-, 2017

25 Komakech R, "A Micropropagation Protocol for the Endangered Medicinal Tree Prunus africana (Hook f.) Kalkman: Genetic Fidelity and Physiological Parameter Assessment" 2020 : 548003-, 2020