분열형 효모에서의 mas2⁺ 유전자의 세포 내 기능

국문 초록 (Abstract)

유전자 발현의 조절은 세포주기 조절에 중요한 역할을 한다. 본 연구에서 새로운 mas2? (mitosis associated protein) 유전자는 인간의 SMARCAD1와 상동성을 갖고 분열형 효모인Schizosaccharomyces pombe (S. pombe)에서 gene-specific PCR방법에 의해서 분리하였다. 분리된 유전자는 SNF2 도메인이 위치해 있고, 이것은 염색체 재구성에 관련되어 있다. adh1?을 이용한 mas2? 전사체의 발현량 분석은 mas2?의 발현수준은 S. pombe에서 격막 형성 전에 가장 높았다. mas2? 완전돌연변이의 세포분열은 26와 35℃에서 지연되는 현상이 보였고, 다수의 다중 격막이나 핵분열이 일어나지 않는 세포들을 발견하였다. 세포들을 완전배지인 YES에서 증식을 증가시키기 위해서 배양했을 때, 정상과 다른 형태의 표현형을 가진 mas2? 완전돌연변이 세포들이 증가했다. 이런 표현형들은 mas2? 유전자의 과발현에 의해서 감소하였다. Mas2 단백질은 S. pombe의 핵내에 위치하였다. 이런 결과들은 mas2?가 인간의 SMARCAD1과 상동성을 갖고 있고, 염색체 재구성과 격막 형성 조절에 사용된다는 것을 나타낸다.

다국어 초록 (Multilingual Abstract)

The regulation of gene expression plays an important role in cell cycle controls. In this study, a novel mas2? (mitosis associated protein) gene, a homolog of human SMARCAD1 was isolated and characterized from a fission yeast Schizosaccharomyces pombe (S. pombe) using gene-specific polymerase chain reaction. The isolated gene contained a complete open reading frame capable of encoding 922 amino acid residues with a typical promoter, as judged by nucleotide sequence analysis. It was also found that an SNF2 domain is located, which is involved in the chromosome remodeling. The quantitative analysis of the mas2? transcript against adh1? showed that the expression level of mas2? is high before septum formation in S. pombe. When mas2? null mutant cells were grown at 27 and 35℃, the cytokinesis of mas2? null mutant was greatly delayed and a large number of multi-septate and mis-segregated cells were produced. In addition, the number of multi-septate cells significantly increased. When cells were cultured in YES rich medium to increase proliferation, the abnormal phenotypes mas2? null mutant dramatically increased. These phenotypes could be rescued by an overexpression of the mas2? gene. The Mas2 protein localized in the nuclei of S. pombe, as evidenced by Mas2-EGFP signals. These results suggest that the mas2? is homologous to human SMARCAD1 gene and involved in septum formation and chromosome remodeling control.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 결과
• 고찰
• 요약
• 감사의 글
• References

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