Fusobacterium nucleatum의 유황화합물 생성에 영향을 미치는 인자 = Factors Affecting the Production of Sulfur Compounds by Fusobacterium nucleatum

다국어 초록 (Multilingual Abstract)

Fusobacterium nucleatum, one of the bacteria causing halitosis, produces the volatile sulfur compounds (VSC) such as H2S in the media containing sulfur components, and forms FeS by binding with iron component. The various factors of oral cavity affect the concentration of sulfur compounds produced by Fusobacterium nucleatum. In this study, the effect of nutrients and pH on the production of sulfur compounds by Fusobacterium nucleatum was studied with the following results;
1. The optical density of broth was increased to 0.817±0.032 and 1.297±0.024 by adding 1.0% sodium thiosulfate and 0.05% L-cysteine hydrochloride in the media, respectively.
2. Though the optical density of broth was 0.799 ± 0.032 by adding volatile sulfur compounds (VSC) only in the media, it was increased to 1.775±0.003 and 1.648±0.022 by adding xylitol combined with glucose and fructose, respectively.
3. The concentration of VSC was above 20,000 ppb in the media above pH 5.5. The optical density of broth was still high in the media with L-cysteine hydrochloride of higher concentration, being low in the media of lower pH.
4. The concentration of VSC was high when there was distilled water or saline solution on the media, and their amount was small.
These results suggest that the production of sulfur compounds by Fusobacterium nucleatum is inhibited by xylitol and acid.