M3HB (Methyl 3-hydroxybutyrate), known as an alternative energy source for cells with impaired metabolicfunction, has recently been reported to have therapeutic effects on Alzheimer’s disease and inhibitionof apoptosis. In this study, the enzymatic ...
M3HB (Methyl 3-hydroxybutyrate), known as an alternative energy source for cells with impaired metabolicfunction, has recently been reported to have therapeutic effects on Alzheimer’s disease and inhibitionof apoptosis. In this study, the enzymatic conversion of M3HB from methanol and betahydroxybutyricacid (BHB) by lipase-catalyzed esterification was performed for the first time. The reactionconditions for maximum M3HB conversion were as follows: Novozym 435, 15 g/L; BHB:methanolmolar ratio, 1:2; reaction temperature, 40 C; and organic solvent, chloroform. The lipase-based M3HBsynthesis achieved about 94.6% conversion through a step-by-step optimization, and is expected to contributeto future bioindustry applications.