Streptomyces nigrifaciens GMT-497이 생산하는 항생물질 MT-497의 분리와 화학적 특성 = Purification and Chemical Characterization of antibiotic MT-497 Produced by Streptomyces nigrifaciens GMT-497

국문 초록 (Abstract)

토양에서 분리한 Streptomyces nigrifaciens GMT-497로부터 G(+) bacteria에만 강한 항균활성을 갖는 항생물질 MT-497을 용매추출, silica column chromatography와 재결정화를 통하여 분리 정제하였다. MT-497의 UV, 융점, 원소분석, IR spectrum, ^1H-NMR과 구성아미노산 분석을 통해 actinocin chromophore와 threonine, proline, methyl valine, sarcosine, aspartic acid로 구성된 prptide을 갖는 actinomycin계열의 항생물질로 동정하였다.

다국어 초록 (Multilingual Abstract)

Antibiotic MT-497 was purified from the culture broth of Streptomyces nigrifaciens GMT-497, which was isolated from soil, by solvent extraction, silica column chromatography and recrystalization. It was confirmed that MT-497 has antibacterial activity against only G(+) bacteria. Through the analysis of UV, melting point, IR spectrum, ^1H-NMR and composition of amino acids, MT-497 was identified as one of the actinomycin antibiotics containing actinocin chromophore and the peptide with threonine, proline, methyl valine, sarcosine and aspartic acid.