Role of mucosal mast cells in visceral hypersensitivity in a rat model of irritable bowel syndrome

다국어 초록 (Multilingual Abstract)

The involvement of mucosal mast cells(MMC) in the pathophysiology of irritable bowel syndrome (IBS) is still controversial. We aimed to re-evaluate the role of MMC invisceral hypersensitivity associated with IBS using a rat IBS model that develops the IBS symptom after a subsidence of acetic acid-induced colitis. No significant difference in the number of MMC was observed betweennormal rat colon and IBS rat colon. (61.7 ± 2.9/mm2 in normal vs. 88.7 ± 13.3/mm2 in IBS, p > 0.29). However, the degranulation rate of MMC was significantly higher inIBS rat colon (49.5 ± 2.4% in normal vs. 68.8 ± 3.4% in IBS, p < 0.05). Pretreatment of a mast cell stabilizer, doxantrazole (5 mg/kg, i.p.), reduced the degranulation rate of MMC and significantly attenuated visceralhypersensitivity to rectal distension in IBS rat, whereas it had no effect on the visceral sensory responses in normal rat. These results suggest that, although the number ofMMC is not significantly changed in IBS rat colon, the higher degranulation rate of MMC is responsible for visceral hypersensitivity in this model IBS.

참고문헌 (Reference)

1 "do they really have a role indisturbed bowel habits of IBS patients?" 96 : 927-929, 2001

2 "Visceral hypersensitivity and altered colonic motility aftersubsidence of inflammation in a rat model of colitis" 9 : 2791-2795, 2003

3 "Therole of psychological and biological factors in postinfectivegut dysfunction" 44 : 400-406, 1999

4 "Terminal ileal mucosal mast cells in irritable bowel syndrome" 38 : 1590-1595, 1993

5 "Symptomsof irritable bowel syndrome in ulcerative colitis in remission" 24 : 190-192, 1983

6 "Stress-induced visceralhypersensitivity to rectal distension in rats:role of CRF andmast cells" 9 : 271-279, 1997

7 "Role of kinin B1 and B2receptors and mast cells in post intestinal infection-inducedhypersensitivity to distension" 10 : 499-508, 1998

8 "Proteinases and proteinase-activated receptor 2:a possiblerole to promote visceral hyperalgesia in rats" 122 : 1035-1047, 2002

9 "Pathobiology of visceral pain: molecularmechanisms and therapeutic implications IV. Visceralafferent contributions to the pathobiology of visceral pain" 278 : 834-838, 2000

10 "Mucosal mast cells (MMC) numbers are normal in biopsiesof patients with irritable bowel syndrome (IBS)" 108 : 860-, 1995

11 "Mast celltryptase regulates rat colonic myocytes through proteinase-activated receptor 2" 100 : 1383-1393, 1997

12 "Mast celldegranulation induces delayed rectal allodynia in rats:role ofhistamine and 5-HT" 43 : 727-737, 1998

13 "Mast cell infiltration anddegranulation in colonic mucosa in the irritable bowel syndrome" 91 : 1675-1676, 1996

14 "Mast Cell-Nerve Interactions" 15 : 213-218, 2000

15 "Management of the irritable bowel syndrome" 120 : 652-668, 201

16 "Intestinal mucosal mast cells in normaland nematode-infected rat intestines are in intimate contactwith peptidergic nerves" 2975-2979,

17 "Increased rectal mucosalenteroendocrine cels, T lymphocytes, and increased gutpermeability following acute Campylobacter enteritis and inpost-dysenteric irritable bowel syndrome" 47 : 804-811, 2000

18 "Increased mast cells in theirritable bowel syndrome" 12 : 449-457, 20

19 "Functional bowel disorders and functional abdominal pain" 45 (45): 43-47, 1999

20 "Fullthicknessbiopsy of the jejunum reveals inflammation and enteric neuropathy in irritable bowel syndrome" 123 : 1972-1979, 2002

21 "Endothelin-1 inducesmucosal mast cel degranulation and tissue injury via ETAreceptors" 103 : 31-34, 202

22 "Distinctive clinical,psychological,and histological features of postinfectiveirritable bowel syndrome" 98 : 1578-1583, 203

23 "Colorectal distension as a noxiousvisceral stimulus:physiologic and pharmacologic characterizationof pseudafective reflexes in the rat" 450 : 153-169, 1988

24 "Altered rectal perception is a biological marker of patientswith irritable bowel syndrome" 109 : 40-52, 195

25 "Activated mast cells infiltrate in close proximity toenteric nerves in diarrhea-predominant irritable bowelsyndrome" 18 : 204-210, 2003

26 "Activated mast cells in proximity to colonic nerves correlatewith abdominal pain in irritable bowel syndrome" 693-702, 204

27 "A role for inflammation in irritable bowelsyndrome?" 51 : 41-44, 2002

28 "A new model ofchronic visceral hypersensitivity in adult rats induced bycolon irritation during postnatal development" 119 : 1276-1285, 2000