곰의말채 추출물과 분리물질의 항산화 및 항주름 활성 = Antioxidation and Anti-wrinkle Activity of Extract Obtained from Cornus macrophyllaWall, and of its Isolated Substance

다국어 초록 (Multilingual Abstract)

To identify new antioxidative and anti-wrinkle ingredients, this study characterized the biological properties, including radical scavenging and elastase inhibition effects, of the crude 80% methanol extract, its subfractions, and the quercetrin isolated from Cornus macrophylla Wall growing in Jeju island. The elastase inhibition activity of the 80% methanol extract and the fractions of both ethyl acetate and butanol showed stronger inhibition activity than Areca catechu extract (used as a reference). The 80% methanol extract and ethyl acetate fraction exhibited free radical scavenging activity on DPPH radical and superoxide with IC50 values of 6.8 μg/mL, 6.2 μg/mL and 1.6 μg/mL, 0.02 μg/mL, respectively, which were stronger than that of butylated hydroxyanisole (9.1 μg/mL) or allopurinol (2.2 μg/mL) as a reference. The ethyl acetate fractions were further purified using a reverse-phase column and prep-HPLC to obtain quercetrin, and its structure was elucidated by NMR. Quercetrin showed significant effects on scavenging DPPH radical and superoxide. These results suggest that the extract of Cornus macrophylla Wall has a considerable potential as a cosmetic ingredient with antioxidative and anti-wrinkle effects. Quercetrin can be used as a functional reference.

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