대두 단백질에 관한 연구 [제2보] : 7S Globulin 중의 복합단백질의 분리 및 그 구성 Subunit 에 대하여 Isolation and Subunit Composition of Multiple 7S Globulins = Studies on Soybean Protein [Part Ⅱ]

다국어 초록 (Multilingual Abstract)

The multiple 7S globulins composed of two fractions (A and B) in the electrophoresis with Davis' method were isolated at different stages of the soybean seed development. Electrophoresis of their subunits liberated in PAWU solvent [phenol-acetic acid-water (2 : 1 : 1) solution plus 5M urea] yielded 4 major bands. Observation of both the electrophoretic bands of the multiple 7S fractions(7S-A and 7S-B) and those of their subunits was suggestive of a similarity of the subunit pattern between two 7S fractions.
The two fractions in multiple 7S globulins were isolated witb DEAE-Sephadex A-50 column(2.0×100㎝) chromatography. They were separated into 2 fractions in a linear gradient concentration of 0.28 to 0.40M NaCl with phosphate buffer (pH 7.8) containing 10mM β-mercaptoethanol(ME).
The isolated protein was dissociated into subunits with two different solvent systems; m PAWU solvent and in Tris-HCl buffer(pH 8.0) containing 1% sodium dodecyl sulfate (SDS) and 40mM ME. The dissociated subunits were subjected to electrophoresis in PAWU-treated 7.5% acrylamide gel and in 1% SDS-treated 5.6% acrylamide gel. In PAWU gel electrophoresis, total 7S globulin was separated into 5 major bands, two of which were occupied in common by two 75 fractions(7S-A and 7S-B). In SDS gel electrophoresis, total 7S globulin was separated into 7 major bands, three of which were overlapped with the subunit of the two 7S fractions.
The above results alluded us to the presence of a common and/or similar subunit between the multiple 7S globulins.