Salvianolic acid B가 자외선B 조사에 의한 plasmid DNA 절단에 미치는 효과 = Effect of Salvianolic acid B on Plasmid DNA Breakage Induced by Ultraviolet B Radiation

다국어 초록 (Multilingual Abstract)

Ultraviolet B (UVB), which is 280-320 nm wavelength light, can induce modification of base and (or) DNA breakage by direct or indirect pathways. Generation of hydroxyl radical is one of the mechanisms by which UVB imparts detrimental effect on DNA. Salvianolic acid B (SalB) is one of the components in Salvia miltiorrhiza and has been reported to have antioxidant, anti-inflammation and anti-wrinkle effects. In the present study, SalB was found to protect the DNA breakage and reduce the 2-deoxy-d-ribose degradation under UVB radiation with H2O2. However, SalB increased the DNA breakage and degradation of 2-deoxy-d-ribose in the presence of Cu2+ ion. These results implied that SalB might have had anti-oxidant or pro-oxidant effect depending on the micro-environment. The current study was carried out with direct UVB radiation to DNA. The roles of SalB in the cell or tissue level still remain to be investigated.

참고문헌 (Reference)

1 이평재, "구리이온(II)이 존재할 때 Salvianolic acid B에 의한 DNA 절단" 대한임상검사과학회 50 (50): 205-210, 2018

2 Kuluncsics Z, "Wavelength dependence of ultraviolet-induced DNA damage distribution : Involvement of direct or indirect mechanisms and possible artefacts" 49 : 71-80, 1999

3 D'Orazio J, "UV radiation and the skin" 14 : 12222-12248, 2013

4 Nagata T, "The mechanism of melanocyte-specific cytotoxicity induced by phenol compounds having a prooxidant effect, relating to the appearance of leukoderma" 2015 : 479798-, 2015

5 Schuch AP, "Sunlight damage to cellular DNA : focus on oxidatively generated lesions" 107 : 110-124, 2017

6 Li X, "Solevent effects and improvements in the deoxyribose degradation assay for hydroxyl radical scavenging" 141 : 2083-2088, 2013

7 이현준, "Salvianolic acid B가 고강도 운동부하에 의한 흰쥐 골격근과 뇌조직의 Oxidative Stress에 미치는 영향" 대한본초학회 31 (31): 99-106, 2016

8 Liu X, "Salvianolic acid B(Sal B)protects retinal pigment epithelial cells from oxidative stress-induced cell death by activating glutaredoxin 1(Grx1)" 17 : 2016

9 Sun Z, "Salvianolic acid B protects normal human dermal fibroblasts against ultraviolet B irradiation-induced photoaging through mitogen-activated protein kinases and activator protein-1 pathways" 91 : 879-886, 2015

10 Amar SK, "Role of type I & type II reactions in DNA damage and activation of caspase 3 via mitochondrial pathway induced by photosensitized benzophenone" 235 : 84-95, 2015

11 Sarwar T, "Redox cycling of endogenous copper by ferulic acid leads to cellular DNA breakage and consequent cell death : a putative cancer chemotherapy mechanism" 289 : 251-261, 2015

12 Yoshino M, "Prooxidant action of gallic acid compounds : copper-dependent strand breaks and the formation of 8-hydroxy-2'-deoxyguanosine in DNA" 16 : 705-709, 2002

13 Cadet J, "Oxidatively generated damage to cellular DNA by UVB and UVA radiation" 91 : 140-155, 2015

14 Hadi SM, "Oxidative breakage of cellular DNA by plant polyphenols : a putative mechanism for anticancer properties" 17 : 370-376, 2007

15 Cavinato M, "Molecular mechanisms of UVB-induced senescence of dermal fibroblast and its relevance for photoaging of the human skin" 94 : 78-82, 2017

16 Patro BS, "Mechanism of coralyne-mediated DNA photo-nicking process" 194 : 140-148, 2019

17 Slominski AT, "How UV light toches the brain and endocrine system through skin, and why" 159 : 1992-2007, 2018

18 Masaki H, "Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation" 206 : 474-479, 1995

19 Audic A, "DNA nicking by ultraviolet radiation is enhanced in the presence of iron and of oxygen" 57 : 508-512, 1993

20 Lloyd RE, "Cu(II)sensitizes pBR322 plasmid DNA to inactivation by UV-B(280-315)" 57 : 1011-1017, 1993

21 Hanqing Pang, "Chemical Analysis of the Herbal Medicine Salviae miltiorrhizae Radix et Rhizoma (Danshen)" MDPI AG 21 (21): 51-, 2016

22 Zhao GR, "Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B" 46 : 73-81, 2008