지질과 phycobiliproteins 고생산성 Arthrospira platensis 변이주 분리

다국어 초록 (Multilingual Abstract)

In this study, microalgae Arthrospira platensis (A. platensis) mutants induced by ethyl methane sulfonate (EMS) and further selection for resistance of cerulenin, a potent inhibitor of fatty acid synthase, were characterized. The mutants selected by 2 μM, 5 μM and 10 μM of cerulenin were designated EC2, EC5 and EC10, respectively. Under normal growth conditions, the mutants and parental strain exhibited similar growth pattern. The mutants of A. platensis showed enhanced lipid accumulation and phycobiliproteins (phycoerythrin, phycocyanin). The lipid content of mutants EC2 and EC5 was about 4.4 and 4.8-fold higher than wild type. The phycoerythrin and phycocyanin content of mutants EC2 and EC5 was increased about 1.5 and 6.9-fold and 1.4 and 3.8-fold, respectively, compared to the wild type. The chlorophyll and carotenoid content of mutants was slightly increased. The high lipid and pigment contents exhibited by A. platensis mutants would make an excellent candidate for the production of commercially interesting biologically active compounds.

목차 (Table of Contents)

• Abstract
• 1. 서론
• 2. 재료 및 방법
• 3. 결과 및 고찰
• 4. 결론
• References

참고문헌 (Reference)

1 Joo, D. S, "Stability of phycocyanin and spectral characteristic of phycobilins from Spirulina platensis" 33 : 482-488, 2000

2 김영민, "Spirulina platensis NIES 39의 성장을 위한 최적배양조건" 한국공업화학회 20 (20): 285-289, 2009

3 Singh, S. C, "Role of lipids and fatty acids in stress tolerance in cyanobacteria" 41 : 297-308, 2002

4 Roman, R. B, "Recovery of pure B-phycoerythrin from microalgae Porphyridium cruentum" 93 : 73-85, 2002

5 Abd, El-Baky H, "Production of carotenoids from marine microalgae and its evaluation as safe food colorant and lowering cholesterol agent" 2 : 792-800, 2007

6 Gill, I, "Polyunsaturated fatty acid: occurrence, biological applications" 15 : 401-409, 2001

7 Chen, W, "Microwave-assisted nile red method for in vivo quantification of neutral lipids in microalgae" 102 : 135-141, 2011

8 Mata, T. M, "Microalgae for biodiesel production and other applications: a review" 14 : 217-232, 2010

9 Gantar, M, "Microalgae and cyanobacteria: food for thought" 44 : 260-268, 2008

10 Heath, R. J, "Lipid biosynthesis as a target for antibacterial agents" 40 : 467-497, 2001

11 Datz, G, "Light dependent changes in the lipid and fatty acid composition of phycocyanin free photosynthetic lamellae of Synechococcus" 69 : 856-862, 1981

12 Fischer, R, "Isolation of mutants, a key for the analysis of complex pathways and for strain improvement, In Microbes for Health, Wealth and Sustainable Environment" Malhortra Publishing House 739-751, 1998

13 Chaturvedi, R, "Isolation of enhanced eicosapentaenoic acid producing mutants of Nannochloropsis oculata ST-6 using ethyl methane sulfonate induced mutagenesis techniques and their characterization at mRNA transcript level" 54 : 208-219, 2006

14 Meireles, L. A, "Increase of the yields of eicosapentaenoic and docosahexaenoic acids by the microalga Pavlova lutheri following random mutagenesis" 81 : 50-55, 2003

15 Carlton, B. C, "Gene mutation, In Manual of Methods for General Bacteriology" American Society for Microbiology 85-96, 1981

16 Solovchenko, A. E, "Effects of light and nitrogen starvation on the content and composition of carotenoids of the green microalga Parietochloreis incisa" 53 : 455-462, 2008

17 Carvalho, A. P, "Effect of culture media on production of polyunsaturated fatty acids by Pavlova lutheri" 21 : 59-71, 2000

18 Bhat, V. B, "C-Phycocyanin: a potent peroxyl radical scavenger in vivo and in vitro" 275 : 20-25, 2000

19 Harun, R, "Bioprocess engineering of microalgae to produce a variety of consumer products" 14 : 1037-1047, 2010

20 Schoefs, B, "Astaxathin accumulation in Haematococcus requires a cytochrome P450 hydroxylase and an active synthesis of fatty acids" 500 : 125-128, 2001

21 Shukla, S. P, "An assessment of biopotential of three cyanobacterial isolates from antarctic for carotenoid production" 40 : 362-366, 2003

22 Saitoh, S, "Aberrant mitosis in fission yeast mutants defective in fatty acid synthetase and acetyl CoA carboxylase" 134 : 949-961, 1996

23 Wang, J, "A quick isolation method for mutants with high lipid yield in oleaginous yeast" 25 : 921-925, 2009