To study the correlation between virulence and Congo red binding capacity of Shigella, 2 invasive strains of Shigella flexneri to HeLa cell were subjected to investigate the regulation of levels of membrane proteins mediated by Congo red.
Two tested ...
To study the correlation between virulence and Congo red binding capacity of Shigella, 2 invasive strains of Shigella flexneri to HeLa cell were subjected to investigate the regulation of levels of membrane proteins mediated by Congo red.
Two tested strains were pigmented on the agar plate containing Congo red.
Virulent S. flexneri grown in the presence of Congo red at 37℃ showed increased levels of three proteins with molecular weight of 43,000, 58,000, 63,000 daltons in the sodium lauryl sarcosinate(SLS) soluble membrane fractions and those proteins were also found in the total membrane fractions. But those proteins were not found in the SLS insoluble fractions.
At 32℃ or 42℃, the protein levels in the SLS soluble fractions or SLS insoluble fractions unaffected by the presence of Congo red.
The expression of these proteins was dependent to the incubation temperature.