Micro‐computed tomography (μ‐CT) scanners permit researchers to characterize the three‐dimensional structure of bone. They allow for the visualization of many bone structural parameters to be quantified with an extremely high degree of precisio...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=O112953680
2020년
-
0892-6638
SCI;SCIE;SCOPUS
학술저널
1-1 [※수록면이 p5 이하이면, Review, Columns, Editor's Note, Abstract 등일 경우가 있습니다.]
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
Micro‐computed tomography (μ‐CT) scanners permit researchers to characterize the three‐dimensional structure of bone. They allow for the visualization of many bone structural parameters to be quantified with an extremely high degree of precisio...
Micro‐computed tomography (μ‐CT) scanners permit researchers to characterize the three‐dimensional structure of bone. They allow for the visualization of many bone structural parameters to be quantified with an extremely high degree of precision. Immunohistochemistry (IHC) (colorimetric) and immunofluorescence (IF) allows for characterization of DNA and RNA in fixed tissue sections. μ‐CT scanners along with IHC and IF allows processed tissue sections to be examined by a variety of different fixatives, in order to characterize the effects of fixation on bone morphology (i.e., adult chicken and mice), and preservation of different types of intact, non‐denatured nucleic acids (e.g., right‐handed B‐DNA, left‐handed Z‐DNA). Fixation was performed using 10% Neutral Buffered Formalin (NBF), Formalin‐Alcohol‐Acetic Acid (FAA), Davidson’s fixative, and Carnoy’s solution for 72 hours at room temperature. Both decalcified and non‐decalcified bone tissues were processed. Ultra‐pure molecular biological grade fixatives and water were used in order to ensure the best histotechnological data. Tissues processed in FAA resulted in hardening of tissue, but good fixation of intact DNA. Tissues processed in 10% NBF resulted in softer tissue, but poor fixation of intact DNA and required antigen retrieval (AR). Tissues processed in Carnoy’s solution resulted in hardened tissue, but excellent DNA IHC and IF. Tissues processed in Davidson’s fixative resulted in hardened tissues, but good preservation of DNA. We will try to correlate structural bone differences observed with the μCT scanner, relative to DNA isolation and IHC and IF staining of bone tissue sections with anti‐B‐DNA and anti‐Z‐DNA monoclonal antibodies. These results will better enable researchers to histotechnologically process tissue while obtaining data on DNA structure and function.
The Role of tRNA Derived Small RNAs in Gene Regulation in Normal Tissues and Cancer
Diversity of Natural Chemicals Based on Variable Environmental Conditions: Discovery and Development
Modulation of Angiotensin Converting Enzyme 2 Activity by Ubiquitination