난자의 회수시기, BSA, Cysteine 및 Myoinositol이 개 난자의 체외성숙에 미치는 영향 = Effect of Ovarian Estrus Stage of Oocytes and Supplementation of Medium with BSA, Cysteine and Myoinositol on in vitro Maturation of Canine Oocytes

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate the effects of ovarian estrus stage of oocytes and supplementation of medium with BSA, cysteine and myoinositol on in vitro maturation of canine oocytes. The in vitro maturation(IVM) rate to metaphase II (M II) stage of oocytes recovered from different stage of the reproductive cycle (inactive, follicular and luteal stage) cultured in TCM-199 media were 0.0±0.0%, 10.0±4.1% and 5.7±1.6%, respectively. The IVM rate of oocytes collected from follicular stage was significantly higher in inactive or luteal stage of oocytes. The IVM rates of oocytes recovered from different stage of the reproductive cycle cultured in TCM-199 media with 5% BSA and 0.1 mM cysteine were 0.0+0.0%, 15.8+4.7%, 5.6+1.5%, respectively. The IVM rates of oocytes recovered from different stage of the reproductive cycle cultured in TCM-199 media with 5% BSA and 10 mM myoinositol were 0.0±0.0%, 18.4±4.6% and 5.7±1.9%, respectively. The IVM rate of follicular stage oocytes was significantly higher in oocytes collected from follicular stage and with cultured 5% BSA and 0.1 mM cysteine or 5% BSA and 10 mM myoinositol compared to other experimental group.