단층형 혈당 바이오센서 개발

다국어 초록 (Multilingual Abstract)

In this research, in order to make up for the shortcomings of the existing 3-layer glucose biosensor, a one-layer glucose biosensor was developed. The one-layer glucose biosensor was formed by using conductive Carbon/Silver Ink on PET film, and the sensor electrode was formed by the Screen-Printing method. In order to set the position of the enzyme and for the protection of the electrode, after formation by using an insulator as well as the Screen-Printing method, dispensing of the enzyme was performed. Fabrication was done at 60℃ for 15 minutes through a drying process to fix the enzyme. After bonding the lower layer (on the electrode formed base electrode film) with the middle layer (creation of a capillary tube to determine the blood amount and to introduce blood), the enzyme was dispensed, and after the dispensed enzyme dried, it was bonded to the top layer (creation of an air vent when the blood was introduced). Compared to the complicated fabrication process of the existing 3-layer structured glucose biosensor, through a very easy fabrication process, a biosensor was fabricated whereby the lower film, through only two printing processes, generally had all the functions of the existing 3-layer structured top-lower-bottom layer biosensor. The result made possible a reduction in sensor production costs and the securing of the reproducibility of the fabrication process. Also, a GDH-FAD (Glucose dehydrogenase flavin adenine dinucleotide) based enzyme compound was applied to improve problems originating from the oxygen interference reaction in GOD (Glucose Oxidase), which is generally used as a glucose biosensor enzyme. Other potential interference during glucose measurement was minimized, and an excellent glucose biosensor with 0.99 % linearity, and possible Hematocrit use range of 30 ~ 60 %, and a CV (Coefficient of variation) of 3.1 % was fabricated. The coefficient of variance (CV) of the sensor developed in this research was slightly unsatisfactory compared to top existing market products, but it falls in the range of ± 15% of the international standard accuracy for glucose measurements, there was little influence of the Hematocrit, and due to the rapid cost reduction due to a decrease in processing, people from low-income and developing countries who manage their blood sugar can do so through low costs. It is expected that it will help in maintaining healthy lives.

목차 (Table of Contents)

• 제 1 장 서론 1
• 1.1 연구배경 및 연구의 필요성 1
• 1.2 바이오센서의 개요 7
• 1.3 혈당측정법의 종류와 원리 10
• 제 2 장 단층형 혈당 바이오센서의 구조 및 원리 13
• 2.1 단층형 혈당 바이오센서의 구조 13
• 2.2 단층형 혈당 바이오센서 전극의 구조 14
• 제 3 장 단층형 혈당 바이오센서의 제작 16
• 3.1 단층형 혈당 바이오센서의 제작 16
• 3.1.1 Screen-Printing을 통한 전극 인쇄 공정 16
• 3.1.2 효소의 제작 18
• 3.1.3 효소의 분주 및 고정화 20
• 3.1.4 건조 및 안정화 22
• 제 4 장 측정 및 고찰 23
• 4.1 측정시스템 23
• 4.2 성능 평가용 검체 25
• 4.2.1 글루코오스 수용액 제조 26
• 4.2.2 헤마토크릿 수치를 조절한 정맥혈 제조 27
• 4.2.3 목표 혈당수치의 정맥혈 제조 30
• 4.3 성능 평가 32
• 4.3.1 센서의 선형성 및 재현성 평가 32
• 4.3.2 센서의 분해능 및 인가전압 평가 36
• 4.4 전극의 헤마토크릿에 대한 영향 40
• 4.5 센서의 온도에 의한 영향 44
• 제 5 장 결 론 46
• 참 고 문 헌 48
• Abstract 50
• 감사의 글 52