간염 B 형 바이러스 표면항원 대장균 내 발현 = Expression of Hepatitis B Virus Surface Antigen Gene in E . Coli

다국어 초록 (Multilingual Abstract)

Three different HBsAg sequences encoding for S region, pre-S2 plus S region, and pre-S2 fused with a portion of β-galactosidase have been expressed in E. coli under the control of lac promoter. When the S region containing HBsAg genes were expressed constitutively in E. coli HB101, the growth inhibition of recombinant cells was observed. However, the constitutive expression of pre-S2 fused with apeptide (N-terminal portion of β-galactosidase) was not detrimental to the E. coli HB101 cells. The antigenicity of the hybrid pre-S2 protein seems to be conformation-independent: treatment of 2-mercaptoethanol or 2-mercaptoethanol/SDS did not abolish the antigenicity.