RAPD 분석을 이용하여 지황 육성 계통과 지역 수집종 들을 구분할 수 있는 분자표지자를 선발하고, 집단 간, 집단 내 유전적 다양성을 평가하기 위하여 본 실험을 수행하였다. 총 20개의 임의 p...
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https://www.riss.kr/link?id=A76265714
2008
English
KCI등재
학술저널
435-440(6쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
RAPD 분석을 이용하여 지황 육성 계통과 지역 수집종 들을 구분할 수 있는 분자표지자를 선발하고, 집단 간, 집단 내 유전적 다양성을 평가하기 위하여 본 실험을 수행하였다. 총 20개의 임의 p...
RAPD 분석을 이용하여 지황 육성 계통과 지역 수집종 들을 구분할 수 있는 분자표지자를 선발하고, 집단 간, 집단 내 유전적 다양성을 평가하기 위하여 본 실험을 수행하였다. 총 20개의 임의 primer를 이용하여 PCR 한 결과, 육성 계통과 수집종 들을 구별할 수 있는 OPA-1 등 10개의 재현성과 다형성이 좋은 프라이머 들을 선발하였다. 특히 OPA-10, OPA-11 및 OPA-19는 고려지황과 지황1호를 다른 계통 및 수집종 들과 구별할 수 있었으며, 이들 프라이머를 이용하여 0.9 kb, 1.2 kb, 1.3 kb 및 1.4 kb 등의 육성계통 특이적인 DNA 밴드들을 확보할 수 있었다. 한편 이들의 결과를 토대로 통계처리에 의한 유전분석 결과, 고려지황, 지황1호 및 일본지황은 집단 내 유사도가 높아 다른 집단들과 구별되었다. 결론적으로, RAPD 분석을 통한 결과는 지황의 유전적 다양성 이해와 특정 계통을 다른 계통 및 수집종 들과 구분할 수 있는 방법으로 이용될 수 있다.
다국어 초록 (Multilingual Abstract)
Random amplified polymorphic DNA (RAPD) markers were used to identify the genetic diversities among and within varieties and landraces of Rehmannia glutinosa. Polymorphic and reproducible bands were produced by 10 primers out of total 20 primers used ...
Random amplified polymorphic DNA (RAPD) markers were used to identify the genetic diversities among and within varieties and landraces of Rehmannia glutinosa. Polymorphic and reproducible bands were produced by 10 primers out of total 20 primers used in the experiment. In RAPD analysis of the 11 genotypes, 64 fragments out of 73 amplified genomic DNA fragments were polymorphic which represented an average 6.4 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPA-1) to 13 (OPA-11) and varied in size from 200 bp to 1,400 bp. Especially, OPA-10, OPA-11 and OPA-19 primers showed specific bands for varieties of Korea Jiwhang and Jiwhang il ho, which could be useful for discriminating from other varieties and landraces of R. glutinosa. Percentage polymorphism ranged from a minimum of 50% (OPA-1) to a maximum of 100% (OPA-11), with an average of 87.7%. Similarity coefficients were higher in the genotypes of Korea Jiwhang and Jiwhang il ho than in other populations. In cluster analysis, genotypes of Korea Jiwhang, Jiwhang il ho, and Japanese accession were separated from those of other varieties and landraces. Average of genetic diversity within the population (HS) was 0.110, while average of total genetic diversity (HT) was 0.229. Across all RAPD makers the GST value was 0.517, indicating that about 52% of the total genetic variation could be explained by RAPDs differences while the remaining 48% might be attributable to differences among samples. Consequently, RAPD analysis was useful method to discriminate different populations such as domestic varieties and other landraces. The results of the present study will be used to understand the population and evolutionary genetics of R. gllutinosa.
목차 (Table of Contents)
참고문헌 (Reference)
1 Tomoda, M., "Water soluble constituents of Rehmannia glutinosa Lib" 19 : 2411-2419, 1971
2 Lewontin,R.C, "The apportionment of human diversity" 6 : 381-394, 1972
3 Korea Food & Drug Administration, "The Korean herbal pharmacopoeia" 129-, 2002
4 Huff, D. R., "RAPD variation within and among natural populations of outcrossing buffalo grass (Buchloe dactyloides)" 86 : 927-934, 1993
5 Cheng, J. L., "RAPD analysis on different varieties of Rehmannia glutinosa" 27 : 505-508, 2002
6 Yeh, F. C., "POPGENE, the user-friendly shareware for population genetic analysis. Version 1.21. Molecular Biology and Biotechnology Centre University of Alberta Canada"
7 Nei, M, "Mathematical model for study the genetic variation in terms of restriction endonucleases" 74 : 5267-5273, 1979
8 Zietkiewicz, E., "Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification" 20 : 176-183, 1994
9 Soller, M, "Genetic polymorphism in varietal identification and genetic improvement" 67 : 25-33, 1993
10 Bornet, B., "Genetic diversity in European and Argentinean cultivated potatoes (Solanum tuberosum sub. tuberosum) detected by inter-simple sequence repeats (ISSRs)" 45 : 481-484, 2002
1 Tomoda, M., "Water soluble constituents of Rehmannia glutinosa Lib" 19 : 2411-2419, 1971
2 Lewontin,R.C, "The apportionment of human diversity" 6 : 381-394, 1972
3 Korea Food & Drug Administration, "The Korean herbal pharmacopoeia" 129-, 2002
4 Huff, D. R., "RAPD variation within and among natural populations of outcrossing buffalo grass (Buchloe dactyloides)" 86 : 927-934, 1993
5 Cheng, J. L., "RAPD analysis on different varieties of Rehmannia glutinosa" 27 : 505-508, 2002
6 Yeh, F. C., "POPGENE, the user-friendly shareware for population genetic analysis. Version 1.21. Molecular Biology and Biotechnology Centre University of Alberta Canada"
7 Nei, M, "Mathematical model for study the genetic variation in terms of restriction endonucleases" 74 : 5267-5273, 1979
8 Zietkiewicz, E., "Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification" 20 : 176-183, 1994
9 Soller, M, "Genetic polymorphism in varietal identification and genetic improvement" 67 : 25-33, 1993
10 Bornet, B., "Genetic diversity in European and Argentinean cultivated potatoes (Solanum tuberosum sub. tuberosum) detected by inter-simple sequence repeats (ISSRs)" 45 : 481-484, 2002
11 Nei,M, "Estimation of average heterozygosity and genetic distance from a small number of individuals" 89 : 583-590, 1978
12 Cao, Z. L., "Effects of radix Rehmanniae and processed radix Rehmanniae on some immune functions in mice" 13 : 22-24, 1988
13 Tsumura, Y., "Diversity and inheritance of inter-simple repeat polymorphism in Douglas fir (Pseudotsuga menziesii) and Sugi (Crypromeria japonica)" 92 : 40-45, 1996
14 Williams, J. G. K, "DNA polymorphism amplified by arbitrary primers are useful as genetic markers" 18 : 6531-6535, 1990
15 Paterson, A. H., "DNA markers in plant improvement" 46 : 39-90, 1991
16 Zhou, Y. Q., "Assessment of genetic diversity of Rehmannia glutinosa germplasm detected by RAPDs and ISSRs" 26 : 922-928, 2004
17 Stewart, C. N, "Assessing population genetic structure and variability with RAPD data: application to Vaccinum macrocarpon (American cranberry)" 9 : 153-171, 1996
18 Lynch, M, "Analysis of population genetic structure with RAPD markers" 3 : 391-399, 1994
19 Wu, K. S, "Abundance, polymorphism and genetic mapping of microsatellites in rice" 24 : 225-235, 1993
20 Vos, P., "AFLP: A new technique for DNA fingerprinting" 23 : 4407-4414, 1995
21 Dellaporta, S. L, "A plant DNA minipreparation, version II" 1 : 19-25, 1983
22 Prevost, A, "A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars" 98 : 107-112, 1999
다중 관계 그래프를 이용한 유전체 보존영역의 계층적 시각화와 개략적 전사 annotation 도구
Advances of Genome Research in Livestock Animals
한국인의 비소세포폐암종에서 O⁶-methylguanine-DNA methyltransferase (MGMT)의 발현도 분석
사람에 존재하는 phosphatase인 chronophin의 환원된 상태에서의 구조
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2027 | 평가예정 | 재인증평가 신청대상 (재인증) | |
2021-01-01 | 평가 | 등재학술지 유지 (재인증) | |
2018-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2015-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2011-08-03 | 학술지명변경 | 외국어명 : Korean Journal of Life Science -> Journal of Life Science | |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2003-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2001-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.37 | 0.37 | 0.42 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.43 | 0.43 | 0.774 | 0.09 |