Trimethyltin에 의한 뇌 손상 마우스에서 galectin-3의 면역조직화학적 발현 조사

다국어 초록 (Multilingual Abstract)

Trimethyltin (TMT), which is an organotin compound with neurotoxicant effects selectively localized in the limbic system (especially, hippocampus), produces mental confusion and temporal lobe seizures. Galectin-3 (Gal-3) is a beta-galactoside-binding lectin important in cell proliferation and apoptotic regulation. In this study, I evaluated the temporal expression of Gal-3 in the hippocampus of adult BALB/c mice after TMT treatment (i.p., 2.5 mg/kg). Western blot analysis showed that Gal-3 immunoreactivity began to appear within 2 days post-treatment; the immunoreactivity significantly peaked within 4 days post-treatment, but the immunoreactivity declined between 4 and 8 days post-treatment. Immunohistochemistry revealed that Gal-3 expression was very rare in the hippocampi of vehicle-treated controls. However, Gal-3 immunoreactivity was obviously appeared 2 and 4 days after TMT treatment and primarily localized in the hippocampal DGs, in which neuronal degeneration occurred. Further, the immunoreactivity was detected predominantly in most of Iba1 (Ionized calcium binding adaptor molecule 1)-positive microglia and in some GFAP (Glial fibrillary acidic protein)-positive astrocytes of the hippocampal DGs. Therefore, Gal-3 significantly increased in activated microglia and astrocytes in response to TMT treatment, indicating that Gal-3 may be involved in pathological processing of neurodegenerative disorder induced by organotin exposure.

목차 (Table of Contents)

• Abstract 1
• Introduction 3
• Materials and Methods 5
• Results 12
• Discussion 27
• References 31
• Abstract in Korean 36