Effects of Needle Electrode electrical stimulation on rat brain by Ischemia-induced Common Carotid Artery Occlusion

다국어 초록 (Multilingual Abstract)

The aim of this study to investigate the effects of needle electrode electrical stimulation (NEES) on ischemia-induced Common Carotid Artery Occlusion. This Ischemia model was used rat brain that completely occluded with non-absorbent suture thread and kept for 5 minutes. Then suture were removed to allow the reperfusion of blood. The test groups were divided into three; the control group without common carotid artery ligation, the GI group in which global ischemia was induced through occlusion of common carotid artery and the NEES group in which NEES used after the global ischemia. NEES was conducted at the meridian points called Choksamni (ST 36) at 12, 24, 48 hours after blood reperfusion. The immune-reactive cells both Bax and caspase-3 indicates the apoptosis were high in the group GI comparing to NEES group. Cox-2 and IL-6 indicates the inflammation were high in the GI comparing to NEES groups. These results suggest that the NEES treatment has the beneficiary effects on ischemia.

목차 (Table of Contents)

• 재료 및 방법
• 결과
• 고찰
• 참고문헌

참고문헌 (Reference)

1 Du, C., "Very delayed infarction after mild focal cerebral ischemia a role for apoptosis" 16 : 195-201, 1996

2 Krajewski, S., "Upregulation of bax protein levels in neurons following cerebral ischemia" 15 (15): 6364-6376, 1995

3 Hinkle, L., "The direction of growth of differentiating neurons and myoblasts from frog embryos in an applied electric field" 314 : 121-135, 1981

4 Zhou, Q., "Target protease specificity of the viral serpin CrmA, Analysis of five caspases" 272 : 7797-7800, 1997

5 Kim, J.S., "Serial Measurement of Interleukin-6 Transforming Growth Factor-b and _100 protein in patients with acute stroke" 27 : 1553-1557, 1996

6 Graham, S.H., "Programmed cell death in cerebral ischemia" 21 : 99-109, 2001

7 Wang, S.J., "Potentiation of Akt and suppression of caspase-9 activations by electroacupuncture after transient middle cerebral artery occlusion in rats" 331 (331): 115-118, 2002

8 Patel, N., "Orientation of neurite growth by extracellular electric fields" 2 (2): 483-496, 1982

9 Srinivasula, S.M., "Molecular ordering of the Fasapoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmAinhibitable protease that activates multiple Ced-3/ICE-like cysteine protease" 14486-14491, 1996

10 Weisz, G., "Modification of in vivo and in vitro TNF-α IL-1 and IL-6 seretion by circulating monocytes during hyperbaric oxygen treatment in patients with perianal Crohn's disease" 17 : 154-158, 1997

11 Hara, A., "Immunohistochemical detection of BAX and Bcl-2 proteins in gerbil hppocampus followiong transient forebrain ischemia" 711 : 249-253, 1996

12 Muzio, M., "FLICE, a novel FADD-homologus ICE/CED-3-like protease, is recruited to the CD95(Fas/APO-1) death-inducing signaling complex" 85 : 817-827, 1996

13 Kim, Y.S., "Electroacupunture-related changes of NADPH-diaphorase and neuronal nitric oxide synthase in the brainstem of spontaneously hypertensive rats" 312 : 63-66, 2001

14 Kerns, J.M., "Electrical stimulation of nerve regerneration in the rat the early effects evaluated by a vibrating probe and elctro-microscopy" 40 : 93-107, 1991

15 Kerns, J.M., "Electrical field effects on crushed nerve regeneration" 117 : 71-80, 1992

16 Tarkwoski, E., "Early intrathecal porduction of interleukin-6 predicts the size of brain lesion in the stroke" 26 : 1393-1398, 1995

17 Yin, W., "Down regulation of COX-2 is involved in hyperbaric oxygen treatment in a rat transient focal cerebral ischemia model" 926 (926): 165-171, 2002

18 Saleh, A., "Cytochrome c and dATP-mediated oligomrization of apaf-1 is a prerequisite for procaspase-9 activation" 274 : 17941-17945, 1999

19 Rao, F.V., "Coupling endoplasmic reticulum stress to the cell death program role of the ER chaperone GRP78" 514 : 122-128, 2002

20 Wu, M.T., "Central Nervous Pathway for Acupunture Stimulation Localization of Procession with Functional MR Imaging of the Brain- Preliminary Experience" 212 : 133-141, 1999

21 Zheng, Z., "Cellular and molecular events underlying ischemia-induced neuronal ap" 16 : 497-503, 2003

22 Gibson, M.E., "BAX contributes to apoptotic-like death following meonatal hypoxia-ischemia evidence for distinct apoptosis pathways" 7 (7): 644-655, 2001

23 Candelario-Jalil, E., "Assessment of the relative contribution of COX-1 and COX-2 iosoforms to ischemia-induced oxidative damage and neurodegeneration following transient global cerebral ischemia" 86 (86): 545-555, 2003

24 Gillardon, F., "Altered expression of BCL-2, BAX, and c-fos colocalizes with DNA fragmentation and ischemic cell damage following middle cerebral artery occlusion in rats" 40 : 245-260, 1996

25 Hitoshi, Y., "A cyclooxygenase-2 inhibitor attenuates spontaneous and TNF-α-induced nonrapid eye movement sleep in rabbits" 285 : R99-R109, 2003