The Effects of Hyaluronic Acid-Carboxymethylcellulose Membrane (GUARDIX-MB®) Barriers on Prevention of Post-operation Peritoneal Adhesions in Dogs

다국어 초록 (Multilingual Abstract)

The aim of this study was to determine the effectiveness of hyaluronic acid-carboxymethylcellulose
membrane (GUARDIX-MB®) barriers on prevention against post-operative peritoneal adhesions. In this study, fourteen
mongrel dogs were divided into two experimental groups: 0.1 % hyaluronic acid (0.1HA) group and hyaluronic acidcarboxymethylcellulose
membrane (HA-CMC) group. In order to induce adhesions, the anti-mesenteric serosa of the
ileum was exteriorized and then abraded in a standard manner by scraping with a scalpel blade to create homogenous
petechial hemorrhagic surface over a 1× 1 cm area. Solution of 0.1HA were simply coated over the abraded tissues,
1.5× 1.5 cm HA-CMC membrane was placed over the abraded tissues, allowed to spread across the intra-abdominal
organs before closure of the abdomen. On day 1 before and day 1, 4, 7, 14, and 21 after operation, venous blood
specimens were collected for measurement of fibrinogen and total WBC. The adhesions were blindly assessed 3 weeks
later by using a computerized tensiometer. The fibrinogen and total WBC values of two groups showed no statistical
significances. The mean tensile strength (gram force, gf) of formed adhesions day 21 after surgery was 88.1± 55.70
gf in the 0.1 % HA group and 24.8± 22.69 gf in the HA-CMC group. The tensile strength values of adhesion separation
HA-CMC membrane group was significantly lower than the 0.1HA group (p<0.05). Therefore, we suggest that HACMC
membrane reduce peritoneal adhesions may be applicable to preventing post-operative intraperitoneal adhesions
in dogs.

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