肝吸蟲症 診斷에 있어서 間接螢光抗體 反應(IFAT)의 應用에 관한 硏究 = The use of IFAT in the diagnosis of human clonorchiasis

다국어 초록 (Multilingual Abstract)

Human clonorchiasis is one of the most common trematode disease and of very important public health problem in Korea. A number of serological techniques have been in use for diagnosing parasitic diseases, efforts in recent years have been made for the development of a more new promising serological technique.
The present study was conducted to evaluate to evaluate the diagnostic potentialities of human clonorchiasis by the indirect fluorescent antibody test (IFAT) using frozen sectioned antigen of adult worm of Clonorchis sinensis. A total of 117 sera from clonorchiasis patients, 16 sera from persons with other parasitic infections and 9 sera from non-infected healthy persons were tested. Frozen sections of adult worm of C. sinensis were placed on the spot-slide and incubated in two fold serially diluted sera and subsequently stained with goat anti-human immunoglobulin labeled with fluorescein isothiocyanate and Evans blue. The worm sections were examined with a fluorescent microscope, A.P\O. Series No. 10 Microstar. If the test is negative, the preparations are colored a uniform red. On ? other hand, if the test is positive, the adult worm sections display an intense green fluorescence localized in the tegumental region of C. sinensis.
The results obtained are as follows:
1. The results indicated that IFA titers at 1/16 were regarded as positive reaction. Therefore, the positive reaction revealed in 72 (61.5%) out of 117 sera from clonorchiasis patients.
2. In the intensity of Clonorchis infection, the positive reactions of IFA titers showed 28.1% in light infection, 68.9% in moderate infection, 77.8% and 84.6% in heavy and very heavy infection respectively.
3. All serum samples from 16 cases infected with parasites other than Clonorchis were diagnosed as negative. Only one out of 9 sera from non-infected healthy cases showed false positive.
With the above results, IFAT has great practical advantage since the antigen is easy to place on slides and easier reading than those of adult C. sinensis particles as an antigen.
Therefore, this study suggest tat the IFAT can be applied in the diagnosis of human clonorchiasis.