RISS 검색 - 국내학술지논문 상세보기

부가정보

다국어 초록 (Multilingual Abstract)

Objective: In intraabdominal abscess induced by Bacteroides fragilis, the infected tissues are characterized by an infiltration of inflammatory cells such as neutrophils and monocytes. To suppress or prevent the formation of abscess, it may be important to activate the inflammatory cells. The purpose of this study was to estimate effects of the combination of antimicrobial drug and immune lymphokines on the treatment of intraabdominal abscesses.
Methods: Immune lymphokines were derived from mitogen-activated splenic lymphocytes of B57BL/6 mice previously immunized against encapsulated B. fragilis. The lymphokines were freed of all substances with the molecular weight less than 1,000 dalton and concentrated to 5-folds using ultrafiltration. The abscess-inducing agents including B. fragilis were injected intraperitoneally into C57BL/6 mice. Therapy of the infected animals started from 5th day after inoculation with the agents. The abscess-bearing mice were treated with the immune lymphokines and/or clindamycin twice a day for 7 days.
Results: 1) The combination of clindamycin and the immune lymphokines showed rather high cure rate (70%). However, the cure rates were less than 20% in the group of clindamycin plus lymphokines prepared from non-immuned spleen cells, the clindamycin alone group or the lymphokines alone group. 2) The efficacy of immune lymphokines on abscess clearance was de-pendent on the initiation time of therapy. 3) The molecular weight of abscess-curing components in the lymphokines derived from the mitogen-activated splenic lymphocytes of mice previously immunized against B. fragilis was estimated to be 10,000 - 100,000 dalton. 4) No growth inhibitory factor of B. fragilis was found in the immune lymphokines.
Conclusion: These results suggest that immunoadjuvant therapy may be more effective than single antibiotic theranv for the treatment of B. fragilis infection.