Effect of Fermentation Conditions on L-Lactic Acid Production from Soybean Straw Hydrolysate = Effect of Fermentation Conditions on L-Lactic Acid Production from Soybean Straw Hydrolysate

다국어 초록 (Multilingual Abstract)

Four types of straw, namely, soybean, wheat, corn, and rice, were investigated for use in lactic acid production. These straws were mainly composed of cellulose, hemicellulose, and lignin. After pretreatment with ammonia, the cellulose content increased, whereas the hemicellulose and lignin contents decreased. Analytical results also showed that the liquid enzymatic hydrolysates were primarily composed of glucose, xylose, and cellobiose. Preliminary experiments showed that a higher lactic acid concentration could be obtained from the wheat and soybean straw. However, soybean straw was chosen as the substrate for lactic acid production owing to its high protein content. The maximum lactic acid yield (0.8 g/g) and lactic acid productivity (0.61 g/(l/h)) were obtained with an initial reducing sugar concentration of 35 g/l at 30°C when using Lactobacillus casei (10% inoculum) for a 42 h fermentation period. Thus, the experimental results demonstrated the feasibility of using a soybean straw enzymatic hydrolysate as a substrate for lactic acid production.

참고문헌 (Reference)

1 Nakasaki K, "Use of wastewater sludge as a raw material for production of Llactic acid" 33 : 198-200, 1999

2 Xu Z, "The study of the ammonia pretreatment effect on the enzyme hydrolysis of soybean straw to produce sugar" 18 : 773-776, 2004

3 Huang Q, "The selection of lignin-degrading fungus and the straw fermentation by mixed strains" 28 : 66-70, 2008

4 Xu Z, "Study on conditions of solid substrate fermentation and enzymolysis of soybean stalk" 24 : 107-110, 2004

5 John RP, "Simultaneous saccharification and fermentation of cassava bagasse for L-(+)-lactic acid production using Lactobacilli" 134 : 263-272, 2006

6 Xu Z, "Production of lactic acid from soybean stalk hydrolysate with Lactobacillus sake and Lactobacillus casei" 42 : 89-92, 2007

7 Tay A, "Production of L(+)-lactic acid from glucose and starch by immobilized cells of Rhizopus oryzae in a rotating fibrous bed bioreactor" 80 : 1-12, 2002

8 Kang KE, "Pretreatment of rapeseed straw by soaking in aqueous ammonia" 35 : 77-84, 2012

9 Kim TH, "Pretreatment of corn stover by soaking in aqueous ammonia at moderate temperatures" 137 : 81-92, 2007

10 Kim TH, "Pretreatment of corn stover by soaking in aqueous ammonia" 124 : 1119-1132, 2005

11 Kim TH, "Pretreatment of corn stover by aqueous ammonia" 90 : 39-47, 2003

12 Gao P, "Preparation of lactic acid, formic acid and acetic acid from cotton cellulose by the alkaline pre-treatment and hydrothermal degradation" 48 : 61-67, 2013

13 Zheng Y, "Overview of biomass pretreatment for cellulosic ethanol production" 2 : 51-68, 2009

14 Zhou Y, "Optimization of L-lactic acid production from glucose by Rhizopus oryzae ATCC 52311" 78 : 401-407, 1999

15 Ding J, "Optimization of L-lactic acid fermentation of corn steep liquor" 32 : 127-130, 2011

16 Yoo CG, "Maximum production of fermentable sugars from barley straw using optimized soaking in aqueous ammonia (SAA) pretreatment" 169 : 2430-2441, 2013

17 Garde A, "Lactic acid production from wheat straw hemicellulose hydrolysate by Lactobacillus pentosus and Lactobacillus brevis" 81 : 217-223, 2002

18 Marques S, "Lactic acid production from recycled paper sludge by simultaneous saccharification and fermentation" 41 : 210-216, 2008

19 Maas RH, "Lactic acid production from lime-treated wheat straw by Bacillus coagulans: neutralization of acid by fed-batch addition of alkaline substrate" 78 : 751-758, 2008

20 Cui F, "Lactic acid production from corn stover using mixed cultures of Lactobacillus rhamnosus and Lactobacillus brevis" 102 : 1831-1836, 2011

21 Sreenath HK, "Lactic acid production from agriculture residues" 23 : 179-184, 2001

22 Sreenath HK, "Lactic acid production by simultaneous saccharification and fermentation of alfalfa fiber" 92 : 518-523, 2001

23 Li Z, "L-Lactic acid production by Lactobacillus casei fermentation with corn steep liquor-supplemented acid-hydrolysate of soybean meal" 1 : 1453-1458, 2006

24 Kou X, "Enzymatic saccharification and L-lactic acid fermentation of corn stover pretreated with liquid hot water by Rhizopus oryzae" 8 : 4899-4911, 2013

25 Xu Z, "Enzymatic hydrolysis of pretreated soybean straw" 31 : 162-167, 2007

26 Nakasaki K, "Effects of intermittent addition of cellulase for production of L-lactic acid from wastewater sludge by simultaneous saccharification and fermentation" 82 : 263-270, 2003

27 Hujanen M, "Effect of temperature and various nitrogen sources on L (+)-lactic acid production by Lactobacillus casei" 45 : 307-313, 1996

28 Bulut S, "Effect of different carbon sources on L(+)-lactic acid production by Rhizopus oryzae" 21 : 33-37, 2004

29 Moldes AB, "Complete bioconversion of hemicellulosic sugars from agricultural residues into lactic acid by Lactobacillus pentosus" 135 : 219-227, 2006

30 Jin AX, "Comparative characterization of degraded and non-degradative hemicelluloses from barley straw and maize stems: composition, structure, and thermal properties" 78 : 609-619, 2009

31 Wang Q, "Bioconversion of kitchen garbage to lactic acid by two wild strains of Lactobacillus species" 40 : 1951-1962, 2005